摘要
以紫果西番莲种籽油粕为研究对象,利用超声微波双辅助法提取油粕中的黄酮,研究乙醇体积分数、料液比、超声功率、超声时间、微波功率、微波时间6个因素对黄酮提取率的影响,结果表明,超声时间、乙醇体积分数、料液比、微波功率对黄酮提取率影响极显著,超声功率、微波时间对黄酮提取率影响显著。采用响应面分析法优化提取工艺参数为:乙醇体积分数73.78%、料液比1∶56.02(g/m L)、超声功率300 W、超声时间50 min、微波功率160 W、微波时间90 s,该条件下黄酮提取率为117.53 mg/g,比常用的乙醇浸提法提高14.81%。
The seed oil residue of Passiflora edulis Sims was used as the study object,and the flavonoids in the seed oil residue were extracted by ultrasonic-microwave-assisted methodology. First single-factor experiments were taken,such as ethanol concentration,solid-to-solvent ratio,ultrasonic power,ultrasonic time,microwave power and microwave time. The results showed that ultrasonic power and microwave time had a significant effect on the extraction rate of flavonoids;ultrasonic time,ethanol concentration,solid-to-solvent ratio and microwave power had a more significant effect on the extraction yield of flavonoids. Response surface methodology （RSM） was applied to optimize the extraction result and a maximum extraction yield of total flavonoids was achieved. The optimal extraction conditions were 73.78% of ethanol concentration,1∶56.02（g/m L） of ratio between solid and liquid,300 W of ultrasonic power,50 min of ultrasonic time,160 W of microwave power,90 s of microwave time,by which the yield of flavonoids reached 117.53 mg/g. The extraction yield of flavonoids by ultrasonic-microwave-assisted methodology is 14.81% higher than that of ethanol extraction.
作者
李焱
黄苇
卢明剑
何颖诗
何子晴
LI Yan;HUANG Wei;LU Ming-jian;HE Ying-shi;HE Zi-qing(College of Food Science,South China Agricultural University,Guangzhou 510642,China;College of engineering,South China Agricultural University,Guangzhou 510642,China)
出处
《广东农业科学》
CAS
2018年第4期132-139,共8页
Guangdong Agricultural Sciences
基金
广东省现代农业产业技术体系创新团队项目(2017LM2151)
关键词
西番莲种籽
油粕
黄酮
超声微波双辅助法
响应面
Passiflora edulis Sims
seed oil residue
flavonoids
ultrasonic-microwave-assisted methodology
response surface methodology
作者简介
李焱（1994-）,女,在读硕士生,E-mail：116129246＠qq.com;通讯作者：黄苇（1967-）,女,硕士,教授,E-mail：weih007＠scau.edu.cn
