摘要
目的探讨定心藤总黄酮对高脂血症大鼠的降血脂作用。方法选择雄性SD大鼠60只,分为6组:正常对照组(NC组)、模型对照组(MC组)、辛伐他汀阳性对照组(PC组)、定心藤总黄酮低剂量组(MIF1组)、定心藤总黄酮中剂量组(MIF2组)、定心藤总黄酮高剂量组(MIF3组)。NC组给予基础饲料;MC组、PC组和MIF各亚组给予高脂饲料,造模同时給予药物干预。喂养6周后,禁食12h,一侧眼球摘除法取血,分离血清,测定血清总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C);喂养12周后,禁食12h,心脏采血,分离血清,检测血清超氧化物歧化酶(SOD),丙二醛(MDA),总抗氧化能力(T-AOC)的水平。结果MIF能够降低高脂血症大鼠血清TC、TG、LDL-C、MDA和升高HDL-C、SOD、T-AOC的水平,与NC组相比,MC组TG、TC、LDL-C、MDA的水平明显升高,HDL-C、SOD、MDA的水平明显降低(P<0.05);与MC相比,MIF各亚组TC、TG、LDL-C、MDA的水平明显降低,HDL-C、SOD、T-AOC的水平明显升高(P<0.05)。结论MIF可能通过提高机体SOD、T-AOC和降低MDA的水平,达到降血脂的作用。
Objective To explore effect of decreasing blood lipid by mappianthus iodoies flavone in rats model. Methods We selected sixty healthy male SD rats,which were randomly divided into six groups .. normal control group （NC） ,model control group （MC）, simvastatin group positive control group （PC）, low dose group of mappianthus iodoies flavonoids （MIF1 group）, middle dose group of mappianthus iodoies flavonoids （MIF2 group）,high dose group of mappianthus iodoies flavonoids （MIF3 group）. After 6 weeks,absolute diet 12 h,the rats of blood samples were drawn from orbit and it was collected to detect serum total cholesterol （TC）, serum triglyceride（TG）, low-density lipoprotein cholesterol （LDL-C）, high density lipoprotein cholesterol （HDL-C）. After 12 weeks, absolute diet 12 h, get blood from heart, serum, centrifugal separation measure serum SOD, MDA content, test the content of T-AOC. Results Mappianthus iodoies flavonoids can decrease the level of TG,TC,LDL-C,MDA and improve HDL-C,SOD and T-AOC in lipid of lipidemia rats. Especially compared with the NC group,the level of TG,TC,LDL-C,MDA in MC group were in- creased,and HDL-C,SOD,T-AOC level were decreased in rats fed high fat diet（P〈0.05）, meanwhile the levels of TC, TG and LDL-C in the treatment groups were decreased, and HDL-C levels were increased（P〈0.05） ;MIF group,which are compared with MC, the level of TC, TG, LDL-C and MDA weresignificantly decreased and the level of HDL-C, SOD, T-AOCwere increased signifi- cantly（P〈0.05）. Conclusion Mappianthus iodoies flavone may improve the level of SOD,T-AOC and decrease the level of MDA to decrease lipid.
出处
《重庆医学》
CAS
北大核心
2017年第4期433-435,438,共4页
Chongqing medicine
基金
广西科学研究与技术开发计划项目(桂科能159832-46
桂科合14123001-22)
桂林市科学研究与技术开发计划项目(20150102-8
20150102-7
20150105-6
20140105-11
20140122-5
20140105-5)
广西植物功能物质研究与利用重点实验室开放基金项目(FPRU2015-5)
关键词
定心藤总黄酮
高脂血症
抗氧化剂
动脉粥样硬化
mappianthus iodoies flavonoidsl hyperlipidemia
antioxidant
atherosclerosis
作者简介
杨光（1988-），在读硕士，主要从事冠心病的基础与临床研究。
通信作者，E-mail：gushengjiu@163．com。
