摘要
目的:建立大黄总蒽醌胶囊中5种蒽醌苷元及5种游离蒽醌的高效液相色谱法含量测定方法。方法:采用Alltima C18柱,(4.6mm×250mm,5μm)色谱柱,流动相:甲醇-0.1%磷酸溶液(85∶15),检测波长:440nm。结果:芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚等5种游离蒽醌线性关系良好,水解后5种蒽醌苷元及总游离蒽醌回收率为100.54%和101.58%,RSD分别为1.4%和1.1%。结论:本方法操作快速、准确,可作为大黄总蒽醌胶囊中蒽醌的质量控制方法。
Objective： To establish an HPLC method for the determination of 5 hydrolysis anthraquinone glycosides and 5 free anthraquinones in rhubarb total anthraquinones capsule by HPLC. Method： Alltima C18column（ 4. 6mm × 200 mm,5μm） was used in HPLC with mobile phase methanol-0. 1% phosphoric acid solutions（ 85 ∶ 15）,the detection wavelength at 440 nm. Results： the calibration curve was good for the aloe-emodin,rhein,emodin,chrysophanol,physcione content. The average recovery ratio of 5 hydrolysis anthraquinone glycosides and 5 free anthraquinones were 100. 54% and 101. 58%. The relative standard deviation were 1. 4% and 1. 1%. Conclusion： This method is rapid and accurate. It can be used for quality control on anthraquinones of Rhubarb total anthraquinones capsule.
出处
《江西中医药大学学报》
2016年第1期68-70,共3页
Journal of Jiangxi University of Chinese Medicine
关键词
大黄总蒽醌胶囊
5种蒽醌苷元
游离蒽醌
总蒽醌
高效液相色谱
Rhubarb Total Anthraquinones Capsule
5 Hydrolysis Anthraquinone Glycosides
Free Anthraquinones
Total Anthraquinones
HPLC
作者简介
吴迪（1980-）女，助理研究员。研究方向：药物研发。Tel：0791—88102554，E—mail：wudigong2011@163．com.
