摘要
目的建立HPLC法同时测定消炎止痛洗剂中大黄酚和盐酸小檗碱的含量。方法色谱条件:采用C18(4.6mm×250mm,5μm)色谱柱,流动相:混合流动相:A为甲醇,B为乙腈,C为0.15mol/L磷酸二氢钠溶液,梯度洗脱,流速:1.0ml/min,柱温:35℃,检测波长:258nm。结果在0.6826~4.3815μg范围内,大黄酚的进样量与峰面积呈良好的线性关系;盐酸小檗碱在0.0838~0.5028μg范围内,盐酸小檗碱的进样量与峰面积呈良好的线性关系;大黄酚的平均回收率为100.20%(RSD=1.15%),盐酸小檗碱的平均回收率为100.33%(RSD=0.96%)。结论该方法操作简单,准确、分离效果好,专属性强、灵敏度高,可以用于消炎止痛洗剂的质量控制。
Objective A method for simultaneous determination of Berberine hydrochloride and Baicalin in Xiaoyan Zhitong Lotion was established. Methods The chromatographic conditions were as follows: The chromatographic column: C18,Mixed mobile phase: The mobile phase A:methanol,B:acetonitrile, C: 0.15 mol/L Sodium dihydrogen phosphate solution, gradient elution was performed, the flow rate:1.0 mL/min, and column temperature:35 ℃,the detection wavelength:258 nm. Results Berberine hydrochloride showed a good linear relationship within 0.6826 ~4.3815 μg, and Chrysophanol within 0.0838 ~0.5028 μg. The average recovery of chrysophanol was 100.20%(RSD = 1.15%),and The average recovery of Berberine hydrochloride was 100.33%(RSD = 0.96%). Conclusion This method was simple, accurate, good separation effect, strong specificity, high sensitivity, and can be used for the quality control of Xiaoyan Zhitong Lotion.
作者
朱云
ZHU Yun(Department of Pharmacy, Lianyungang affiliated Hospital, Nanjing University of traditional Chinese Medicine, Lianyungang, Jiangsu 222002, China)
出处
《中国处方药》
2019年第7期48-49,共2页
Journal of China Prescription Drug
