摘要
目的：观察唐古特大黄多糖（ＴＭＰ）对小鼠急性肝损伤的保护作用及探讨ＴＭＰ对肝损伤的保护机理．方法：小鼠６０只，随机分６组，生理盐水正常对照组，药物模型组，生理盐水模型对照组和３个给药组，每组按要求分别用ＣＣｌ４１５６ｍｇ／ｋｇ，Ｄ－Ｇａｌ－Ｎ８００ｍｇ／ｋｇ，ＴＡＡ５５０ｍｇ／ｋｇ腹腔注射（ｉｐ），造成小鼠急性肝损伤，以ＴＭＰ１００ｍｇ／ｋｇ，２００ｍｇ／ｋｇ，４００ｍｇ／ｋｇ×５ｄ灌胃，用硫代巴比妥酸比色法测定小鼠肝组织ＭＤＡ含量，用比色法测定小鼠血清的ＡＬＴ，ＡＳＴ含量以观察ＴＭＰ对小鼠急性肝损伤的保护作用．结果：ＴＭＰ灌胃保护，肝组织ＭＤＡ，血清ＡＬＴ，ＡＳＴ含量明显低于对照组（Ｐ＜０．０５），且呈剂量依赖性．结论：ＴＭＰ对ＣＣｌ４，Ｄ－Ｇａｌ－Ｎ，ＴＡＡ引起的小鼠急性肝损伤有明显的保护作用．
AIM: To investigate the effects of TMP on acute liver injury in mice and to study the protective mechanism of TMP in mice with acute liver injury. METHODS: Three acute liver injury models of mice were made by using CCl4 (156 mg/kg), DGalN (800 mg/kg) and TAA (550 mg/kg) ip respectively, following TMP 100 mg/kg, 200 mg/kg and 400 mg/kg ig for 5 days respectively. RESULTS: The increasing trend of AST and ALT in serum and MDA in liver tissue was significantly inhibited. CONCLUSION: TMP can protect mice against CCl4, DGalN or TAAinduced acute liver injury.
出处
《第四军医大学学报》
1999年第6期549-551,共3页
Journal of the Fourth Military Medical University
关键词
唐古特大黄多糖
肝损伤
保护作用
中药
TMP
acute liver injury model
mice
liver diseases, traditional Chinese medicine
