摘要
目的建立与人类发病机理相似的大鼠口腔癌前病变与鳞癌模型,为进一步研究口腔鳞癌的发生发展机制提供实验对象。方法在屏障环境下将质量分数为0.004%的4-硝基喹啉-1-氧化物(4-nitro-quinoline-1-oxide,4NQO)水溶液,作为饮水给予大鼠自行饮用,观察大鼠口腔黏膜的变化,并在第10、14、18、22、24周分5批处死,采集口腔黏膜标本,确定病理分级及病变性质。结果 82只有效实验大鼠中:1舌正常黏膜25.6%(21/82)、上皮单纯增生25.6%(21/82)、轻度异常增生17.1%(14/82)、中度异常增生9.8%(8/82)、重度异常增生2.4%(2/82)、鳞癌19.5%(16/82);2腭正常黏膜20.7%(17/82)、上皮单纯增生23.2%(19/82)、轻度异常增生24.4%(20/82)、中度异常增生14.6%(12/82)、重度异常增生7.3%(6/82)、鳞癌9.8%(8/82)。实验组病理分级结果采用等级资料的多个独立样本的Kruskal-Wallis秩和检验,舌黏膜(χ2=20.738,P<0.005)、腭黏膜(χ2=15.003,P<0.005),结果分布差异均有统计学意义。结论该模型可排除其它外界因素对实验结果的干扰,实验效果明确,是理想的研究口腔鳞癌发生发展机制的动物模型。
Objective To establish the oral precancerous and oral squamnus cell carcinoma models which the patho- genesis is similar to human＇ s and provide experimental subjects for further study on the mechanism of occurrence and de- velopment of oral squamous cell carcinoma. Methods In harrier environment, 4NQO aqueous solution with the mass fraction of 0. 004% was given to rats as water to drink. Then changes of oral mueosa in rats were observed. The rats were divided into 5 groups and each group of them was killed respectively in the 10th week, 14th week, 18th week, 22th week and 24 th week. Oral mucosa specimens were collected and pathological classification was done to determine the nature of the lesions. Results Eighty-two cases of rats： ① Tongue nnnnal mucosa 25.6% （21/82）, epithelial hyperplasia 25.6% （21/82） , mild dysplasia 17.1% （ 14/82 ） , moderate dysplasia 9.8% （ 8/82 ） , severe dysplasia 2.4% （ 2/82 ） and squamous cell carcinomal 9.5% （16/82）; ② palatal normal mucosa 20.7% （17/82）, epithelial hyperplasia 23.2% （19/82）, mild dysplasia 24.4% （20/82） , moderate dysplasia 24.6% （12/82）, severe dysplasia 7.3% （6/82） and squamous cell eareinoma 9.8% （8/82）. Distribution differences, experimental pathology classification result was statistically significant （ tongue mucosa X^2 = 20. 738, P 〈 0. 005, palatal mucosal X^2 = 15. 003, P 〈 0. 005 ）. Con- clusion This model is an ideal animal model for study on the mechanism of oral cancer occurrence and developmental process.
出处
《广东牙病防治》
2015年第2期66-70,共5页
Journal of Dental Prevention and Treatment
基金
四川省应用基础研究计划项目基金(2013JY0126)
作者简介
聂敏海E-mail：nieminhai@126．com
