摘要
目的探讨脂肪酸合成酶(FASN)与脂肪细胞型脂肪酸结合蛋白(A-FABP)在乳腺浸润性导管癌(IDC)组织和人乳腺癌细胞系中的表达及其与临床病理学特征的关系,探讨FASN与A-FABP的相关性及其与癌细胞侵袭的关系。方法用免疫组织化学染色方法检测FASN与A-FABP在58例IDC组织及12例正常乳腺组织中的表达分布,根据阳性细胞数百分率及阳性沉淀物的显色程度计算阳性表达百分率;用细胞划痕实验检测SKBR3和MCF-7细胞的迁移侵袭性,并用Western blot方法检测FASN与A-FABP细胞在SKBR3和MCF-7细胞株中的表达差异。结果正常乳腺组织中FASN和A-FABP阳性表达率分别为8.3%(1/12)和16.7%(1/6),58例IDC组织中FASN、A-FABP阳性表达率分别为72.4%(42/58)、79.3%(46/58),FASN和A-FABP在正常乳腺组织中阳性表达率与IDC组织中阳性表达率差异均有统计学意义(P<0.01,P<0.05),FASN和A-FABP在IDC有淋巴结转移、且肿瘤直径>2cm组织中阳性表达百分率较无淋巴结转移、肿瘤直径≤2cm组织中高,差异均有统计学意义(P<0.01);在IDC组中FASN与A-FABP表达具有相关性(r=0.797,P<0.001)。SKBR3细胞迁移率在划痕12、24h高于MCF-7细胞(P<0.05),FASN在SKBR3细胞中表达较MCF-7高。结论 FASN和A-FABP在乳腺浸润性导管癌组织中的表达与淋巴结转移及肿瘤大小有关,且在IDC组织中FASN与A-FABP表达存在相关性。在乳腺癌细胞株中FASN与乳腺癌的侵袭转移有关。
Objective To study the expression of fatty acid synthase（FASN）and adipocyte fatty acid-binding protein（A-FABP）in human infiltrating ductal breast cancer（IDC）tissues and hunman breast cancer cells and the relationship with the clinicopathogical characteristics.To further explore the relationship between FASN and AFABP,and the relevance of the invasion in cancer cell.Methods The expression of FASN and A-FABP was detected in 58 cases of human infiltrating ductal breast cancer and 12 cases of human normal breast tissues by immunohistochemistry technique,calculated positive expression percentage according to the number of positive cells percentage and the staining degree of positive sediment.The cell wound-healing assay was applied to detect the invasion of SKBR3 and MCF-7cells.Western blot was used to detect the expression of FASN and A-FABP in MCF-7and SKBR3 cells.Results The positive rates of FASN and A-FABP were 8.3%（1/12）and 16.7%（1/6）respectively in 12 cases of normal breast tissues by immunohistochemistry.In 58 cases of IDC tissues,the positive rates of FASN and A-FABP were 72.4%（42/58）and 79.3%（46/58）respectively.The differences of the positive rates of FASN and A-FABP in normal breast and IDC tissues were statistically significant（P〈0.01,P〈0.05）.The expression of FASN and A-FABP was up-regulated in the lymph node metastasis positive group and the larger size group（tumor diameter〉2cm）when compared with lymph node metastasis negative group or the diameter≤2cm group,the differences were statistically significant（P〈 0.01）.In IDC group,the expression of FASN correlated with A-FABP（r=0.797,P〈0.001）,The migration rate of SKBR3 was significantly higher than MCF-7cell at 12,24h（P〈0.05）,FASN expression in SKBR3 was higher than that in MCF-7.Conclusion FASN and A-FABP might associated with the lymph node metastasis and tumor size,and there was correlation between FASN and A-FABP in human IDC tissues.FASN may associated with the invasion and metastasis in breast cancer cells.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2015年第2期228-233,共6页
Journal of Sichuan University(Medical Sciences)
基金
国家自然科学基金项目(No.J1103604
No.81272907)
教育部留学回国人员科研启动基金项目(No.2012-1707-7-7)资助
作者简介
通讯作者，E-mail：lihua_scu@scu．edu．cn
