摘要
目的 CD8+T细胞在慢性阻塞性肺疾病患者气道中增多且持续存在,观察CD8+T细胞在烟草烟雾暴露及脂多糖(lipopolysaccharide,LPS)诱导大鼠慢性支气管炎的膜性支气管表达以探讨CD8+T细胞在慢性支气管炎中的作用。方法18只健康雄性Wistar大鼠按随机数字表法分为3组:假吸烟组、慢性支气管炎组、N-乙酰半胱氨酸(acetylcysteine,NAC)组,假吸烟组经气道注入等渗盐水,慢性支气管炎大鼠模型采用2次气管内注入LPS及烟熏4周法制备,NAC组在慢性支气管炎组基础上予NAC(200 mg/kg)灌胃干预。各组大鼠取肺组织HE染色观察膜性支气管的病理形态并进行炎症病变病理评分,核因子-κB(nuclear factorκB,NF-κB)、Ⅰ类主要相容性复合体(major histocompatibility complex class I,MHC I)、CD8+T细胞和血管内皮生长因子(vascular endothelial growth factor,VEGF)免疫组化采用免疫组化SP法染色检测。结果假吸烟组、NAC组病理积分[1.17±2.40、4.66±2.25]均较慢性支气管炎组(10.83±3.31)明显降低(P<0.05),假吸烟组与NAC组病理积分差异无统计学意义(P>0.05)。慢性支气管炎组NF-κB、MHC I、CD8+T细胞、VEGF表达的中位数(4.84、2.48、5.35、5.02)较假吸烟组(1.18、1.25、1.33、1.18)、NAC组(2.18、1.46、2.35、2.02)明显升高(P<0.05),而假吸烟组与NAC组的差异无统计学意义(P>0.05)。慢性支气管炎组NF-κB、MHC I与CD8+T细胞呈正相关(r=0.670,P<0.01;r=0.701,P<0.01),CD8+T细胞与VEGF、小气道病理积分呈正相关(r=0.689,r=0.782)。结论 NAC可能通过抑制小气道上皮组织生成NF-κB、MHC I来减轻CD8+T细胞和VEGF的表达从而减轻小气道炎症。
Objective CD8 ＋T cells increased in the airway of patients with chronic obstructive pulmonary disease and exis -ted constantly .The aim was to investigate the role of CD 8 ＋T-cells in rats with chronic bronchitis （ CB） which was induced by cigarette smoking and intratracheal injection with lipopolysaccharide （ LPS） . Methods 18 health Wistar rats were radomly divided into sham smoking group（group A）, CB group（group B） and N-acetylcysteine prevention group （group C）.The rats in group B and group C re-ceived intratracheal injection with LPS twice and exposed to cigarette smoking for 4 weeks to induce CB model .The rats in Group C re-ceiving intragastric administration with N-acetylcysteine （NAC）（200mg/kg） before received LPS and smoking.Group A was the sham smoking group.The lung tissue of all rats were stained by HE then evaluated about pathological scores .The expression of nuclear fac-tor-κB （NF-κB）, major histocompatibility complex class I （MHCI）, CD8 ＋T cell and Vascular endothelial growth factor （VEGF） in airway were detected by immunohistochemisty which was stained by labeled streptavidin biotin method . Results The pathological scores of airway （ 10 .83 ±3 .31 ） in group B were higher than （1.17 ±2.40） in group A（P 〈0.05）.The pathological scores of airway（4.66 ±2.25） in group C were less than （10.83 ±3.31） in group B（P 〈0.05）.The expression of NF-κB（4.84）, MHC I （2.48）,CD8 ＋T cell（5.35）and VEGF（5.02） in airway increased in group B when compared with （1.18, 1.25, 1.33） and （1.18） in group A respectively（P 〈0.05）.The expression of NF-κB （2.18）, MHC I（1.46）,CD8 ＋（2.35）and VEGF（2.02） in airway decreased＆nbsp;in group C when compared with （4.84）, MHC I（2.48）,CD8 ＋T cell（5.35）and VEGF（5.02） in group B respectively （P〈0.05 ）. There were positive correlations between the expression of NF-κB, MHC I and CD8 ＋T cells in airways（r=0.670, r=0.701, respec-tively, all P〈0.01）.There were positive correlations between the expression of CD 8 ＋T cells and VEGF the pathological scores of air-ways（r=0.689, r=0.782, respectively, all P〈0.01）. Conclusion NAC can inhibit airway inflammation which is regulated by CD8 ＋T-cells and VEGF through suppressing the expression of NF -κB and MHC I.
出处
《医学研究生学报》
CAS
北大核心
2015年第1期16-19,共4页
Journal of Medical Postgraduates
基金
国家自然科学基金(81260013)
作者简介
通讯作者：钟小宁，E—mail：xnzhong101@sina．com
