摘要
目的为了解我院门诊及住院患者梅毒感染情况,探讨梅毒抗体血清学筛查方法甲苯胺红不加热血清试验(Toluidine Red Unheated Serum Test,TRUST)和梅毒螺旋体酶联免疫吸附试验(Enzyne-Linked Immunosorbent Assay with Treponemal Pallidam Antigen,TP-ELISA)联合检测及明胶颗粒间接凝集试验(Particle Agglutination Assay,TPPA)在梅毒筛查中的意义。方法对2012年1月-2013年1月来我院就诊的门诊及住院患者7 072例用TRUST法及TP-ELISA法同步进行梅毒抗体血清学筛查,对检出的阳性标本用明胶颗粒间接凝集试验(Particle Agglutination Assay,TPPA)进行确认。结果 7 072份标本中经TPPA确认试验阳性104例,占同期门诊及住院患者的1.47%。TRUST法灵敏度为69.23%(72/104),假阳性率为5.77%(6/104),漏检率为30.77%(32/104);ELISA法灵敏度为98.08%(102/104),假阳性率为13.46%(14/104),假阴性率为1.92%(2/104)。结论联合应用TRUST和TP-ELISA两种血清学方法可提高梅毒的检出率,应作为产前、术前及输血前梅毒检测的常规筛查方法和策略。对TRUST或TP-ELISA法任何一种试验阳性者均应进行TPPA检测,进一步提高阳性检测结果的可信度,避免医疗纠纷的发生。
Objective To understand the hospital outpatient and hospitalized patients with syphilis infections. To study antibody serological screening methods for syphilis, such as the toluidine red unheated serum test（TRUST） and the treponema pallidum enzyme-linked immunosorbent assay（TP-ELISA） joint detection and gelatin particles indirect agglutination test（TPPA）. Methods Form January 2012 to January 2013, 7072 cases were from the hospital outpatient and hospitalized patients with TRUST and TP-ELISA screening syphilis antibody serology, and the detection of specimens with gelatin particles indirect Agglutination test（TPPA） was conducted for the confirmation. Results In 7072 specimens, using TPPA validation tests, 104 cases were positive,accounting for 1.47% of the outpatient and hospitalized patients. TRUST sensitivity was 69.23%（72/104）. The false positive rate was5.77%（6/104）, and the missing rate was 30.77%（32/104）. For the ELISA method, the sensitivity was 98.08%（102/104）, and the false positive rate was 13.46%（14/104）. The false negative rate was 1.92%（2/104）. Conclusion Combination of TRUST and TP-ELISA methods can improve the detection rate of syphilis. Those methods should be used as routine screening methods for syphilis detection. For the positivity of TRUST or TP-ELISA, TPPA test should be further taken to improve the reliability of positive results, ultimately to avoid the occurrence of medical disputes.
出处
《现代预防医学》
CAS
北大核心
2015年第3期573-574,576,共3页
Modern Preventive Medicine
作者简介
刘厚丽（1973-），女，本科，主管检验师，研究方向：临床免疫学及细胞学
