摘要
目的 建立测定五味沙棘颗粒中异鼠李素含量的高效液相色谱法.方法 采用Kromsail C18柱（250 mm×4.6 mm,5μm）,四氢呋喃-乙腈-0.4％磷酸水溶液（12：18：70）为流动相,进行等度洗脱,检测波长为370 nm,流速为1 mL/min,室温下测定.结果 异鼠李素进样量在0.3～3 μg范围内与峰面积呈良好线性关系（r2=0.999 9）,平均回收率为97.93％,RSD为1.55％（n=6）,检测限为0.031 2 μg/mL.结论 该方法简单准确、重复性好,适用于五味沙棘颗粒中异鼠李素的含量测定.
Objective To establish a high performance liquid chromatography（HPLC） method for the determination of isorhamnetin in Wuwei Shaji Granules.Methods The separation was performed on the Kromsail C18 column （250 mm × 4.6 mm,5 μm） by gradient elution with tetrahydrofuran-acetonitrile-0.4％ phosphoric acid aqueous solution（12：18：70） as the mobile phase.The detection wavelength was 370 nm.The flow rate was 1 mL/min.The detecting was performed under ambient temperature.Results The linear range of isorhamnetin was 0.3-3 μg/mL（r2 =0.999 9）.The average recovery rate was 97.93％,RSD 1.55％ （n =6）.The detection limit was 0.031 2 μg/mL.Conclusion This method is simple and accurate with good repeatability,which is suitable for the isorhamnetin determination in Wuwei Shaji Granules.
出处
《中国药业》
CAS
2013年第21期27-28,共2页
China Pharmaceuticals
关键词
高效液相色谱法
五味沙棘颗粒
异鼠李素
high performance liquid chromatography
Wuwei Shaji Granules
isorhamnetin
作者简介
耿婕（1988-）,女,在读硕士研究生,（电子信箱）254499142@qq.com
薛培凤（1969-）,教授,硕士生导师,博士生副导师,本文通讯作者,（电话）0471-6653146（电子信箱）xpfdc@vip.sina.com.
