摘要
为了筛选紫萼玉簪根多糖的最佳提取条件,并研究紫萼玉簪根多糖抑菌活性,试验采用超声辅助法提取多糖,用硫酸苯酚法测定多糖含量,在料液比、超声次数、超声功率单因素试验的基础上,采用Box-Behnken响应面设计法建立数学模型,以确定最佳提取工艺;并测定紫萼玉簪根多糖对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌的最小抑菌浓度（MIC）。结果表明：紫萼玉簪根多糖在超声辅助提取中最佳提取工艺为超声功率390 W（52%）,料液比1∶93,超声次数128次（6 min 40 s）,多糖得率为23.6%,与理论值相差0.2百分点;紫萼玉簪根多糖对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌的MIC分别为18.75 mg/mL、37.5 mg/mL、75 mg/mL。
In order to select the optimum extraction condition of polysaccharides from the root of Hosta ventricosa and to research its antibacterial activity, ultrasonic-assisted method was used in this experiment for the extraction of palysaccharides,and sulphuric acid-phenol method was adopted for the determination of polysaccharides content. Based on the data of single-factor tests including solid-liquid ratio, ultrasonic frequency and ultrasonic power, Box-Behnken response surface design method was used to establish the mathematical model and to determine optimum ex- traction process. The MIC values of polysaccharides from the root of Hosta ventricosa against Escherichia eoli ,Staphylococcus aureus and Bacillus subtilis were also measured. The results showed that ultrasonic-assisted optimum extraction condition of polysaccharides from the root of Hosta ventricosa was 390 W（ 52% ） for ultrasonic power, 1：93 for solid-liquid ratio, 128 （ 6 min 40 s） for ultrasonic frequency. Polysaccharide yield was 23.6% ,which had 0. 2 difference lower than the theoretical value. The MIC values of polysaccharides from the root of Hosta ventricosa against Escherichia coli,Staphylococcus aureus and Bacillus subtilis were 18.75 mg/mL,37.5 mg,/mL and 75 mg/mL respectively.
作者
黄榕
王衍莉
包桥桥
刘回民
刘树英
刘洪章
HUANG Rong;WANG Yanli;BAO Qiaoqiao;LIU Huimin;LIU Shuying;LIU Hongzhang(Institute of Life Science,Jilin Agricultural University,Changchun 130118,China)
出处
《黑龙江畜牧兽医》
CAS
北大核心
2018年第16期168-172,共5页
Heilongjiang Animal Science And veterinary Medicine
基金
国家科技部成果转化项目(2014GB2B100007)
关键词
紫萼玉簪
超声辅助提取
多糖
响应面法
抑菌活性
Hosta ventricosa
ultrasonic-assisted extraction
polysaccharide
response surface method
antibacterial activity
作者简介
黄榕（1994-），女，硕士研究生，研究方向为植物大分子结构与功能，1421543159@qq.com。;通信作者：刘洪章（1957-），男，教授，博士，博士生导师，研究方向为植物资源，lhz999@126.com．
