摘要
目的研究中国沙棘果实的化学成分,并测定部分化学成分体外活性。方法采用95%乙醇渗滤提得总浸膏,经溶剂萃取及柱层析分离,采用化学及光谱方法进行结构鉴定,并对小鼠视网膜色素上皮细胞(RPE)建立H2O2损伤模型,使用3种单体与细胞共孵育,进行MTT检测分析。结果分得3个新化合物,分别鉴定为异鼠李素-3-O-β-D-(6-O-反式芥子酰基)槐二糖-7-O-α-L-鼠李糖苷(Ⅰ)、山柰素-3-O-β-D-(6-O-反式芥子酰基)槐二糖-7-O-α-L-鼠李糖苷(Ⅱ)和槲皮素-3-O-β-D-(6-O-反式芥子酰基)槐二糖-7-O-α-L-鼠李糖苷(Ⅲ)。化合物Ⅲ对RPE有抗氧化活性,化合物Ⅰ则无,槲皮素活性最强。结论研究结果可为中国沙棘的临床应用提供理论依据。
OBJECTIVE To study the chemical constituents isolated from the berries of sea buckthorn(Hippophae rhamnoides Subsp.Sinensis rousi),and the antioxidation of some constituents in vitro.METHODS The berries were extracted with alcohol.The chemical constituents of the extract were isolated and purified by chromatographic methods.Chemical and spectral methods were used to determine the structures of the purified compounds.The RPE were injured by H2O2.Three compounds were added into the culture medium,and cell via...
出处
《华西药学杂志》
CAS
CSCD
北大核心
2010年第6期633-636,共4页
West China Journal of Pharmaceutical Sciences
基金
国家自然科学基金资助项目(批准号:30472153)
关键词
中国沙棘
化学成分
抗氧化
视网膜色素上皮细胞
Hippophae rhamnoides Subsp.Sinensis rousi
Chemical constituents
Antioxdation
RPE cells
