摘要
目的:研究柚皮苷对体外培养的人牙周膜成纤维细胞(HPDLCs)成骨分化能力的影响。方法:组织块法培养原代HPDLCs,加入含不同浓度柚皮苷的培养液(100、10、1、0.1、0.01 mg/L),用碱性磷酸酶(ALP)试剂盒检测HPDLCs的培养上清液中ALP的活性,用实时定量PCR检测加入柚皮苷后不同时间点(24h、48h、72h),HPDLCs的骨形成蛋白2(BMP2)、I型胶原蛋白(Col I)、骨保护蛋白(OPG)和核因子κb受体活化因子配体(RANKL)的mRNA表达水平的变化。结果:与对照组比较,不同浓度柚皮苷试验组HPDLCs培养上清液中的ALP活性显著增高(P<0.05),其中1mg/L组的ALP活性最高,此浓度柚皮苷作用下,24h后,BMP-2的mRNA表达水平显著升高,并呈时间依赖性,48h后,Col I和OPG的mRNA表达水平也显著上升。结论:柚皮苷促进了HPDLCs细胞向骨细胞分化功能,其作用可能是通过上调Coll I、BMP2和OPG基因的表达。
Objective To study the effect of Naringin on osteogenic differentiation of human periodontal ligament cells （HPDLCs）. Methods Different dosage （100,10,1,0.1,0.01 mg/L） of Naringin were added in the primary cultured HPDLCs from explant culture method.The osteogenic differentiation activities were examined by ALP analysis.The mRNA expression of BMP2,Col I,OPG and RANKL were investigated by means of real-time PCR. Results The administration of Naringin significantly induced the osteogenic differentiation activities with the increased expression level of ALP and 1 mg/L is the most effective dosage. After 24h,the mRNA expression of BMP＇2 significantly increased in the HPDLCs treated by naringin administration,which is time-dependence. After 48h, the mRNA expression of Col I and OPG significantly increased. Conclusion Naringin may induce osteogenic differentiation of HPDLCs by upregulation of the mRNA expression of Col I,BMP2 and OPG.
出处
《中国美容医学》
CAS
2013年第3期355-358,共4页
Chinese Journal of Aesthetic Medicine
基金
广东省中医药管理局资助课题(20111037)
深圳市福田区科技项目(FTWS201103
FTWS201022)
作者简介
刘建林，男，主任医师，主要从事牙周组织改建的生物学机制研究；E-mail：liujianlin999@sina．com
