摘要
目的:探讨骨碎补柚皮苷对人牙周韧带细胞的增殖能力以及成骨分化潜能的影响。方法:MTT法检测在含有不同浓度骨碎补柚皮苷的细胞培养液中人牙周韧带细胞的增殖特性;检测不同浓度骨碎补柚皮苷作用后人牙周韧带细胞中碱性磷酸酶(ALP)活性的改变。结果:骨碎补柚皮苷≥0.01μmol/L时能增进人牙周韧带细胞增殖(P<0.05),并能增加其ALP活性(P<0.05)。结论:骨碎补柚皮苷可提高人牙周韧带细胞的增殖能力及成骨分化的潜能。
Objective： To investigate the effects of Drynaria fortunei J. Smith naringin （DFSN） on proliferation and differentiation of human periodontal ligament cells （hPDLC）. Methods： Cultured human periodontal ligament cells were treated with DFSN at different concentrations. Cell proliferation was determined by MTF method;cell differentiation was evaluated through the examination of alkaline phosphate （ALP） activities. Results： DFSN in a concentration ranged from 0.01 μmol/L to 10 μmol/L showed a promoting effect on proliferation of hPDLC （ P 〈 0.05 ）, and it also promoted ALP activities of hPDLC （ P 〈 0.05 ）. Conclusion ： Drynaria fortunei J. Smith naringin can promotes the proliferation and differentiation of human periodontal ligament cells.
出处
《浙江大学学报（医学版）》
CAS
CSCD
北大核心
2010年第1期79-83,共5页
Journal of Zhejiang University(Medical Sciences)
基金
浙江省科技厅资助项目(991103115)
作者简介
胡其勇（1976-），男，本科，主治医师，从事口腔临床医疗工作．
通讯作者：陈莉丽（1957-），硕士，主任医师，口腔内科工作；E-mail：yanchen_5657@163．com
