摘要
目的对黄芪降压方中两种活性成分进行含量测定,建立活性成分多指标质量控制方法。方法对黄芪降压方提取液进行UHPLC-MS分析,色谱柱为Agilent SB C_(18)(2.1×100mm,2.7μm)柱,流动相采用乙腈-去离子水(60∶40,v/v),测定黄芪甲苷和柚皮苷,采用ESI离子源,检测离子对分别为829/783和579/271。结果制备的10批黄芪降压方中,黄芪甲苷的平均含量为0.1382mg·m L^(-1),柚皮苷平均含量为0.3386mg·m L^(-1)。结论该方法快速、灵敏、准确,适宜用于对黄芪降压方进行活性导向的多指标质量控制。
OBJECTIVE To determine two active compounds with in Huangqi formula of anti-hypertension, and establish effect guided multi-index method for quality control. METHODS UHPLC-MS was used for determination, using Agilent SB C 18 （2. 1 × 100mm, 2.7μm）, and a mobile phase containing acetonitrile-deionized water;the ion source was ESI, the target compounds were astragaloside A and naringin, with the ion respectively. RESULTS the mean content of astragaloside A and naringin was 1. pair of 829/783 and 579/271, 306 and 1. 531mg · g-1 in 10 preparations of Huangqi formula of anti-hypertension. CONCLUSION The method was fast, sensitive and accurate, which was suitable for effective guided multi-index quality control of Huangqi formula of anti-hypertension.
作者
梁妙莲
谭梅英
詹利之
LIANG Miao-lian;TAN Mei-ying;ZHAN Li-zhi(Foshan City TCM hospital Sanshui hospital, Foshan 528100, China;Guangdong Province 2nd Hospital of TCM, Guangzhou 510095, China;Institute of Tropical Medicine, Guangzhou University of Chinese Medicine, Guangzhou .510405, China)
出处
《海峡药学》
2018年第3期23-26,共4页
Strait Pharmaceutical Journal
关键词
黄芪降压方
活性成分
LC—MS
Huangqi formula of anti-hypertension
Active compounds
LC-MS
作者简介
梁妙莲，女（1979．09-）。毕业于广东药学院。职称：主管中药师。从事中药质量标准研究工作。E-mail：Liangmiaolian1123@126.com
