摘要
比较水浴加热、直接加热煮沸、微波辅助浸提3种水浸提取方法,确定明日叶叶总黄酮提取率最高的水浸提工艺条件,得到粗提液,并进一步考察大孔树脂类型、粗提液上样量、乙醇洗脱液pH值、乙醇体积分数、洗脱液用量等条件对水溶性总黄酮提纯结果的影响。结果表明:水浴加热浸提的提取率最高,其最佳浸提条件为水浴温度95℃,加热时间10min。HP-20大孔树脂对明日叶叶中总黄酮具有较好吸附与解吸性能,最佳提纯工艺条件为质量浓度为2.445mg/mL的粗提液上样量为40mL,洗脱液乙醇体积分数70%、pH2、洗脱用量为8BV。提纯产物总黄酮含量比明日叶叶干粉的含量提高了约6.8倍。
In the present study,three aqueous extraction techniques,water bath extraction,direct heating extraction and microwave-assisted extraction were compared to determine the most suitable one for maximum extraction yield of water-soluble flavonoids from Angelica keiskei Koidzumi leaves.The water bath extraction method provided maximum extraction of water-soluble flavonoids and the optimal extraction conditions were heating at 95 ℃ for 10 min.Further investigations were carried to explore the purification of the resulting crude extract under various conditions of macroporous resin type,sample loading amount,and elution solvent（ethanol） pH,ethanol concentration and elution volume.HP-20 type resin was found to have higher adsorption and desorption capacities for flavonoids and the optimal conditions for purifying water-soluble flavonoids from Angelica keiskei Koidzumi leaves using the resin were determined as follows： 40 mL of crude extract sample at 2.445 mg/mL were loaded onto HP-20 type resin and then desorbed with 8 BV of 70% acidified ethanol（pH 2）,resulting in a purification factor ofapproximately 6.8.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第22期27-32,共6页
Food Science
基金
国家自然科学基金青年科学基金项目(31000814
31000800)
国家自然科学基金面上项目(31171642)
科技部农业成果转化项目(2011GB2C000008)
上海市科学技术委员会项目(10DZ0504000
1052NM06700)
上海交通大学医工交叉项目(YG2011MS64)
关键词
明日叶
水溶性总黄酮
大孔树脂
水浸提
提纯
Angelica keiskei Koidzumi
water-soluble flavonoids
macroporous resin
water extraction
purification
作者简介
郭晓青（1964-），女，副教授，本科，研究方向为食品化学。E-mail：gyguoxiaoqing@163．com
通信作者：吴金鸿（1978-），女，助理研究员，博士，研究方向为功能食品和食品加工。E-mail：wujinhong@sjtu．edu．cn
