摘要
通过京西葛茎段的离体培养和快速繁殖实验,探讨了京西葛不定芽的诱导、分化、增殖及生根的最佳培养条件。结果表明:以优良京西葛的具腋芽茎段为外植体,经2.2%NaClO灭菌25～30min后成活率可达50%;适于京西葛不定芽诱导的培养基为MS+NAA0.1mg/L+6-BA0.5mg/L,诱导分化率为88.89%;适于不定芽分化及继代培养的培养基为MS+NAA0.15mg/L+6-BA0.3mg/L+2,4-D0.2mg/L,继代芽分化率为83.33%,继代倍数达6～7倍;适于生根的培养基为1/2MS+NAA0.5mg/L,生根率达100%。本研究最终建立了京西葛的组织培养体系并初步建立起一套较为完善的快速繁殖技术体系。
The optimal tissue culture conditions for induction,differentiation,proliferation and rooting of adventitious buds of Pueraria lobata Ohwi were studied through in vitro culture and rapid propagation technologies.The results showed that using stem segments with axillary buds from elite parent trees of P.lobata as explants,the survival rate could reach 50% after 25-30 min of sterilization with 2.2% NaClO.The most optimal medium for induction of adventitious buds was MS ＋ NAA 0.1 mg/L ＋ 6-BA 0.5 mg/L,and the induction rate reached 88.89%.The most optimal medium for differentiation and subculture of the adventitious buds was MS ＋ NAA 0.15 mg/L ＋ 6-BA 0.3 mg/L ＋ 2,4-D 0.2 mg/L;the differentiation rate reached 83.33%,and the multiples of proliferation rate ranged from 6 to 7.The most optimal rooting medium was 1/2 MS supplemented with NAA 0.5 mg/L,and the rooting rate could reach 100%.Overall,a tissue culture system and a relatively good technological system for rapid propagation of P.lobata were established.
出处
《北京林业大学学报》
CAS
CSCD
北大核心
2010年第5期138-142,共5页
Journal of Beijing Forestry University
基金
“十一五”国家科技支撑计划项目(2009BADA7B04、2008BAD95B12)
关键词
京西葛
茎段
组织培养
不定芽
愈伤组织
Pueraria lobata
stem segment
tissue culture
adventitious bud
callus
作者简介
张晓霞。主要研究方向：药用植物。电话：010—62349042 Email：zxxl216@163．com 地址：100083北京市清华东路35号北京林业大学628信箱。
杨富裕，博士，副教授。主要研究方向：草业科学与生物质能源。电话：010-62733083 Email：yfuyu@263．net 地址：100193北京圆明圆西路中国农业大学动物科技学院。
刘玉军，教授。主要研究方向：药用植物。电话：010-62337855 Email：yjliubio@163．com 地址：100083北京市清华东路35号北京林业大学生物科学与技术学院。
