摘要
为了实现大巴山粉葛Pueraria lobata(wild)Ohwi Dabashan种苗的规模化快速繁殖,以大巴山粉葛茎段为外植体,研究了灭菌时间、MS培养基大量元素、植物生长调节剂对大巴山粉葛组织培养过程中消毒、初代培养、增殖培养及生根培养的影响。结果表明,(1)先用75%乙醇消毒20 s,再用0.1%氯化汞消毒10 min,成活率达67.56%。(2)初代培养基为1/2 MS+0.5 mg·L^(-1)6-BA+0.3 mg·L~(^(-1))IBA,腋芽萌发率可达92.2%;(3)增殖培养基为1/2 MS+1.0 mg·L^(-1)6-BA+0.05 mg·L^(-1)IBA,增殖系数约5.0;(4)生根培养基为1/2 MS+0.01 mg·L^(-1)NAA+0.1 mg·L^(-1)IBA,生根率达96.03%。
In order to realize the rapid propagation of Pueraria lobata（wild） Ohwi Dabashan, the effects of steriliza-tion time, macroelement in MS medium and plant growth regulators on disinfection , initial culture, multiplication culture and rooting culture of Pueraria lobata（ wild） Ohwi Dabashan were studied by tissues culture , using its stem with axillary bud as explants .The results showed that：（1） The explants were sterilized in 75%alcohol and 0.1%HgCl2 for 20 s and 10 min, the survival rate reached to 67.56%.（2） The medium for the initial culture was 1/2 MS＋0.5 mg· L^-1 6-BA＋0.3 mg· L^-1 IBA, the percentage of axillary bud was up to 92.2%.（3） The optimum multipli-cation medium was 1/2 MS＋1.0 mg· L^-1 6-BA＋0.05 mg· L^-1 IBA, multiplication coefficient reached to 5.0.（4） The rooting medium was 1/2 MS＋0.01 mg· L^-1 NAA＋0.1 mg· L^-1 IBA, with rooting rate 96.03%.
出处
《浙江农业学报》
CSCD
北大核心
2016年第7期1108-1114,共7页
Acta Agriculturae Zhejiangensis
基金
四川省育种攻关项目(2011NA0098-10)
关键词
大巴山粉葛
组织培养
腋芽萌发
植物生长调节剂
Pueraria lobata（ wild） Ohwi Dabashan
tissues culture
axillary bud sprouting
plant growth regulator
作者简介
华晓琴（1988-）,女,甘肃武威人,硕士研究生,研究方向为林木遗传育种.E-mail：278689104@qq.com
通信作者,钟宇,E-mail：zhongyu315@163.com
