摘要
选取沙漠乔桑不同的外植体,通过芽的诱导、试管苗的快速增殖、试管苗生根和驯化移栽筛选出适宜的工厂化育苗工艺流程。3—6月份选取沙漠乔桑待萌发冬芽、萌发侧芽,流水冲洗干净后,70%酒精浸10s,然后用0.15%氯化汞灭菌5～10min,以不同组合的培养基对外植体进行诱导、快速繁殖、生根。结果表明:以MS+BA1.0mg/l(单位下同)+NAA0.1作诱导培养基,用MS+BA0.5+NAA0.05作增殖培养基进行快速繁殖,以1/2MS+NAA0.2作生根培养基,移栽到草炭:珍珠岩:蛭石=3:1:2的混合基质中。通过试验筛选出了一套快速繁殖体系,建立了适宜于沙漠乔桑工厂化生产的工艺流程。
The appropriate craft of mass production has been. obtained through the inducement bud and rapid multiplication and rootage and taming to transplant of tube seedling by selecting the different explants. Between March and June, the hibernacula and the lateral bud of the desert tall mulberry has been selected and washed. Then by 70% alcohol immersing them for 10s, putting out the germ with 0.15% Hg2Cl2 for 5-10 minutes, the explants has been induced and rapidly multiplicated and taken root by the different combination of medium. The result showed that ： the most suitable culture medium of bud sprouting is MS＋ BA1.0mg/L＋ the NAA0.1 mg/L. The most suitable multiplication culture medium is MS＋ BA 0.5 mg/L ＋ the NAA 0.05 mg/L. The most suitable rootage culture medium is 1/2 MS＋NAA0.2 mg/L. The suitable transplanting medium is turf： perlite： vermiculite=3：1：2. Through the experiments, a set of rapid multiplication system has been obtained and the craft process of the desert tall mulberry production has been built up.
出处
《中国农学通报》
CSCD
2007年第1期355-358,共4页
Chinese Agricultural Science Bulletin
关键词
沙漠乔桑
组织培养
工厂化生产
工艺流程
The Desert Tall Mulberry, Tissue Culture, The Factory Turns the Production, Craft Process
作者简介
石文山，男，1963年出生，副教授．本科，农业推广硕士。滨州职业学院生物工程系实验实习办主任，主持植物组培中心工作.主要从事植物生理与植物组织培养的教学科研工作。通信地址：256624 山东省滨州市渤海九路北首533号滨州职业学院生物工程系：Tel；0543-3277929：E-mail：wemhanshigg@yahoo．com．cn
