摘要
为了选育高产、优质、多抗的水稻新品种，采用花粉管通道法，将密穗高粱总ＤＮＡ导入水稻晚粳品种鄂宜１０５，从Ｄ１至Ｄ５代变异材料中选育出ＤＨ３，ＤＨ４，ＤＨ５等多个具有高光效、高产和优质的新品系．经随机扩增多态ＤＮＡ技术对供体、受体、后代进行分子验证，后代中出现了在供体中存在而在受体中不存在的泳带．解剖学观察发现了后代剑叶、穗颈及穗基的输导组织比受体发达，维管束数目比受体增加１０％～３０％．后代ＤＨ３，ＤＨ４的光合速率亦显著高于受体．在形态解剖、光合特性和分子水平上进一步证明了高粱ＤＮＡ片段在受体中的表达和稳定遗传．对ＤＨ３，ＤＨ４，ＤＨ５等品系进行生产示范，增产效果显著，单产高达７５００ｋｇ／ｈｍ２．ＤＨ５已于１９９９年２月通过长沙市品种审定，定名为长晚粳１号．米质分析结果表明，整精米率、蛋白质含量。
To select high yield,high quality and high disease resistance rice variety,sorghum total DNA was introduced into later season rice Eyi 105 through rice pollen tube in this experiment. More than 10 desirable lines (DH series) were selected from the variant plants from D 1 to D 5 generations. RAPD analysis showed that some electrophoresis bands were found in the selected lines which was also found in the sorghum supplier but not in Eyi 105 acceptor. Anatomical observation found that the flag leaf and the bottom stem of ear in DH series had about 10%￣30% more well developed vascular bundles than the Eyi 105. Net photosynthesis rates of DH 3 and DH 4 were found significantly higher than Eyi 105. These results suggested that some pieces of sorghum DNA were successfully introduced and expressed in the selected lines at anatomical, physiological and molecular levels. Large scale field demonstrational experiments showed that the selected lines had an advantage of high yield over Eyi 105, the average yield reached 7 500 kg/hm 2. Quality analysis also revealed that the selected lines had better indexes than the national standard for good quality rice. In addition, brewing experiment using DH 5 showed improvement in wine quality because of its high polyphenol content.DH 5 was officially approved to be a new variety in February,1999 and renamed as Changwangeng No 1.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
1999年第2期87-91,共5页
Journal of Hunan Agricultural University(Natural Sciences)
基金
湖南省科委攻关课题
关键词
高粱
DNA导入
水稻
随机扩增多态性
DNA分析
sorghum deoxyribonucleic acid introduce paddy random amplified polymorphic DNA
