摘要
从60对SSR引物中筛选出17对,对供体高粱、受体水稻及其外源DNA导入后代稳定材料进行了分子验证,结果表明供体高粱与受体水稻间存在多态性、受体水稻与后代稳定材料间出现多态性,供体高粱与部分后代稳定材料间出现相同的扩增带,部分供体与受体共有的特征带在后代中消失、而有些后代出现了供体和受体均没有的扩增带。这表明供体高粱的DNA不同片段已整合到水稻品种中,获得了不同的变异材料,这些DNA片段能在后代中稳定遗传。
Sorghum genome DNA had been transfered into rice variety via pollen tube pathway. In this paper, the molecular verification was performed by SSR technique. The result shown seventeen of sixty pairs of primers can reveal polymorphisms among donor, receptor and their stable offspring. Same bandings were amplified between donor and some offspring. Some bandings appeared both donor and receptor, however disappeared offspring, at the same time several bandings could be found based on offspring but not donor or receptor. This suggested sorghum DNA fragment had been integrated into receptor rice, and led to stably inherit.
出处
《分子植物育种》
CAS
CSCD
2004年第4期501-505,共5页
Molecular Plant Breeding
基金
重庆“十五”良种创新工程
重庆市自然基金资助。
