摘要
将含有Camv35S启动子、卡那霉素抗性基因和GUS报告基因,目的基因为GAI基因的转化载体质粒pBI121,通过基因枪轰击巴西橡胶树(Hevea brasiliensis Muell.-Arg.)花药愈伤组织,50 mg L-1卡那霉素的继代培养基进行抗性筛选。获得了抗性再生植株,经过PCR、Southern检测结果表明:GAI基因已经成功转入橡胶树基因组中。
The vector pBIl21 particle, which contains Camv35S promoter, kanamycin resistance gene, GUS reporter gene and GAI gene, was transfered into the Hevea brasiliensis anther callus by particle bombardment. The regenerated plantlets were obtained in the subculture media supplemented with kanamycin of 50 mg L^-1. PCR and Southern Blotting demonstrated that the GAI gene was transferred into the H. brasiliensis genome successfully.
出处
《热带亚热带植物学报》
CAS
CSCD
北大核心
2010年第2期165-169,共5页
Journal of Tropical and Subtropical Botany
基金
国家自然科学基金(30660098)
中国热带农业科学院中央级公益性科研院所基本科研业务费专项(ITBBZD200711)资助
关键词
巴西橡胶树
基因枪
GAI基因
Hevea brasiliensis
Particle bombardment
GAI gene
作者简介
通讯作者Correspondingauthor，email：cxt6988063@163．com
