摘要
为了探讨利用基因工程技术进行橡胶树品质改良的可行性,用基因枪轰击巴西橡胶(Hevea brasiliensis)愈伤组织,将GAI矮化基因导入橡胶受体中,通过50mg L-1卡那霉素筛选鉴定,优化了基因枪法转化橡胶的各种参数。结果表明,DNA金弹与靶细胞的距离为9cm,每皿轰击一次时,胚状体诱导率可以达到1.87%。经过GUS组织染色和PCR扩增鉴定,初步确定GAI基因已经整合到橡胶基因组中。
GAI gene was transferred to calli from anther culture ofHevea brasiliensis by particle bombardment to develop dwarf rubber trees. Plant material was bombarded with plasmid PBI121 containing kanamycin resistant, GUS and GAI genes. Kanamycm concentration at 50 mg L^-1 was used as selection pressure alter concentration gradient experiments. The experiments showed that each plate of Hevea calli, which was bombarded once by gold particles coated with the plasmid and the distance between particles and target cells at 9 cm, had good results, the embryoid induction rate reaching to 1.87%. It was confirmed that GAI gene had been inserted into Hevea genome as detected by histochemical staining using X-gluc and PCR analysis.
出处
《热带亚热带植物学报》
CAS
CSCD
北大核心
2006年第3期179-182,共4页
Journal of Tropical and Subtropical Botany
基金
国家自然科学基金(30460114)
热带作物生物技术国家重点实验室开放资金项目(KL200401)
中国热带农业科学院重点科技项目(Rykj0201)资助
关键词
巴西橡胶
基因枪
GAI基因
遗传转化
Hevea brasiliensis
Particle bombardment
GAI gene
Genetic transformation
作者简介
通讯作者
