摘要
绿色荧光蛋白GFP是植物遗传转化常用的报告基因,但是有些植物组织由于具有绿色荧光背景而不能使用。为了探索GFP基因作为报告基因在巴西橡胶树遗传转化中利用的可行性,对巴西橡胶树不同的离体材料进行荧光背景检测,发现花药愈伤组织、未授粉胚珠愈伤组织、游离小孢子、花药壁体细胞均能发出极强的绿色荧光,叶片侧脉细胞发出弱的绿色荧光,而叶肉细胞不能发出绿色荧光。这表明橡胶树愈伤组织、游离小孢子、花药壁体细胞、叶片侧脉细胞含有自发荧光物质,在以它们为受体构建转化体系时不能以GFP基因作为报告基因。
Green fluorescent protein （GFP） gene is a widely used report gene in plant genetic transformation, but may not be used when the specific plant tissues have inner green fluorescent background. To explore the feasibility of GFP gene as a reporter in the transformation in rubber tree, fluorescence background of different cultures from the rubber tree was studied. It was found that calli from anther and non-pollinated ovule, isolated microspores, and somatic cells of anther wall emitted strong green fluorescence; lateral vein cell emitted weak green fluorescence; mesophyll cell did not issue green fluorescence. Results indicated that the calli, the isolated microspores, the somatic cells of anther wall and the lateral vein cell contained auto-fluorescent substances. The GFP gene can not be used as the reporter gene when they are used as receptor gene to construct transformation system.
出处
《热带作物学报》
CSCD
2008年第1期23-26,共4页
Chinese Journal of Tropical Crops
基金
国家自然科学基金资助(30260062
30660152)项目
中央级公益性科研院所基本科研业务费专项
作者简介
谭德冠，电话：0898—66984866，E-mail：tdg@scuta．edu.cn
张家明,通讯作者。
