摘要
构建了同时含有番茄几丁质酶基因(Chi3)和β-1,3-葡聚糖酶基因(Glu-Ac)的双价抗真菌基因植物表达载体,以西瓜(Citrulluslanatus)子叶块为外植体,采用根癌农杆菌(Agrobacteriumtumefaciens)介导法,将Chi3和Glu-Ac同时导入西瓜栽培种“中育一号”,共获得46株抗性再生植株。经PCR、Southernblot和RT-PCR检测,表明外源基因已成功整合到西瓜基因组中,并在转录水平得到表达。利用尖孢镰刀菌西瓜专化型(Fusariumoxysporum)对转基因植株进行离体叶片抗病性检测,表明转基因植株对枯萎病的抗性均有不同程度的增强。
Using plant bivalent expression vector pCAMBIA1300 and pBI121 as basic components, a new binary vector harboring tomato chitinase gene Chi3 and tomato β-1,3-glucanase gene Glu-Ac, was constructed through sequential restriction digests and ligations recombination. The two genes driven by CaMV35s promoter were successfully transferred into watermelon cultivars “ZhongYuYiHao” via Agrobacterium-mediated transformation. Successful integration and expression of Chi3 and Glu-A c were confirmed by PCP,Southern blot analysis and RT-PCR. The reaction of detached leaves of transgenic plants to infection Fusarium oxysporum infection was examined. The results indicated that transgenic plants gained certain resistance.
出处
《热带亚热带植物学报》
CAS
CSCD
北大核心
2005年第5期369-374,共6页
Journal of Tropical and Subtropical Botany
基金
福建省自然科学基金项目(B0510017)资助
作者简介
通讯作者
