摘要
建立发光酶免疫分析(LEIA)方法进行环孢霉素(Cyclosporine)治疗药物监测(TDM)。用定标、质控、回收率、精密度、抗干扰等对LEIA进行评价,并对用药达稳态浓度后肾移植患者的肝素抗凝全血中的环孢霉素血药浓度进行测定。结果表明:定标、质控均符合实验要求,平均回收率为99.6%,CV为2.1%,高胆红素、血红蛋白、甘油三酯以及高、低总蛋白等对LEIA的干扰误差均<10%。肾移植患者无药物中毒反应和排斥反应,环孢霉素血药浓度平均值为190.3μg/L。LEIA法测定环孢霉素血药浓度具有较高的特异性和稳定性。
To set up the luminescence enzyme immunoassay （LEIA） for therapeutic drug monitoring of cyclosporine, LEIA was evaluated by the index of calibration, quality control, recovery, yield, precision and anti-interference. The blood concentration of cyelosporine in patients with the kidney transplant was quantitatively measured by LEIA. The results showed that the calibration and quality control results by LEIA were in accord with standard. The average recovery, yield was reached 99.6% and the CV of LEIA was 2. 1 %. The interference error of high bilirubin, hemoglobin, triglycerides, total protein, and low total protein to LEIA was less than 10%. There was no drug toxicity and exclude reaction in the patients with kidney transplant, and the average blood cyclosporine concentrations in these patients was 190.3 μg/L. The LEIA has high accuracy, specificity and stability in the measurement of blood cyclosporine concentration.
出处
《标记免疫分析与临床》
CAS
2008年第5期299-302,共4页
Labeled Immunoassays and Clinical Medicine
关键词
发光酶免疫分析
治疗药物监测
环孢霉素
Luminescence enzyme immunoassay
Therapeutic drug monitoring
Cyclosporine
