摘要
目的:采用聚甲基丙烯酸丁酯(PBMA)整体柱毛细管电色谱法测定冬虫夏草中胞苷和腺苷的含量。方法:采用自制的PBMA电色谱整体柱(总长为34.5 cm,有效长度26.0 cm),流动相20 mmol.L-1硼砂水溶液(pH 3.5),运行电压15kV,压力进样(6×105)Pa×0.1 m in;柱温30℃,检测波长214 nm,内标溶液100μg.mL-1甲氧苄啶(TMP)溶液(乙醇-流动相1∶1为溶剂)。结果:两核苷的在12.5~125 mg.L-1线性关系良好。检测限(S/N=3)及定量限(S/N=10)分别为:胞苷2.14,7.14 mg.L-1;腺苷1.88,6.25 mg.L-1。两核苷加样回收率在97.2%~103.5%,RSD在0.9%~2.6%。结论:该方法可用于冬虫夏草中胞苷和腺苷的含量测定。
Objective: To develop a capillary electrochromatography method for determination of cytidine and adenosine in cordyceps with monolithic column.Method: The total length of the home-made ploy-butyl methacrylate(PBMA) monolithic capillary electrochromatographic column was 34.5 cm with the effective length of 26.0 cm.The mobile phase was 20 mmol·L-1 borax solution(adjusted pH to 3.5 using acetic acid);the operation voltage was 15 kV;sample injection pressure was 6 bar×0.1 min;column temperature was 30 ℃ and the...
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第5期587-590,共4页
China Journal of Chinese Materia Medica
关键词
整体毛细管电色谱法
冬虫夏草
胞苷
腺苷
含量测定
monolithic capillary electrochromatography
cordyceps
cytidine
adenosine
determination
