摘要
目的:观察中药黄芩苷对脂多糖(lipopolysaccharide,LPS)介导体外培养的人牙周膜细胞(periodontal ligament cell,PDLC)增殖的时间效应和细胞周期的影响。方法:采用细胞培养技术培养PDLC,应用噻唑盐比色测定法(MTT)和流式细胞仪技术测定黄芩苷对LPS介导PDLC增殖的时间效应和细胞周期。结果:加入0.01μg/ml黄芩苷3 h后,明显促进人牙周膜细胞的增殖(P<0.05);加入100μg/ml LPS 3 h后,即明显抑制人牙周膜细胞的增殖(P<0.05),抑制作用随时间的延长而增强,而同时加入0.01μg/ml黄芩苷和100μg/ml LPS3 h后,其抑制作用则显著减弱(P<0.05)。加入0.01μg/ml黄芩苷后12 h,DNA合成前期细胞所占百分比(G1%)较对照组降低,而DNA合成期细胞所占百分比(S%)较对照组增高;加入100μg/ml LPS后12 h,G1%较对照组增高,而S%则降低;加入0.01μg/ml黄芩苷和100μg/ml LPS后12 h,G1%较LPS组显著降低(P<0.01),S%则有显著提高(P<0.01)。结论:中药黄芩苷能显著促进PDLC增殖分化,对LPS抑制PDLC的活性有保护作用,原理可能是LPS抑制静止态的G1期细胞进入S期,从而抑制细胞的增殖;而黄芩苷则相反促进细胞由G1期细胞进入S期,从而促进细胞的增殖。
Objective：To observe the effects of Chinese herb baicalin on proliferation and cell cycle of periodontal ligament cells （PDLC） by stimulation of lipopolysaccharide （LPS） in vitro. Methods：Human PDLC was cultured in vitro. MTT colormetic method was used to evaluate the effects of Chinese herb baicalin on proliferation of PDLC by stimulation of LPS. Flow cytometry was used to analyze the possible DNA index and cellular cycle changes of PDLC. Results：PDLC showed a significant growth inhibition after being treaded with 100 μg/ml LPS 3 h by MTT methods, and proliferation ability of PDLC improved greatly after adding baicalin. FCM results showed that the cell number increased in G1 phase, while the cell number decreased in S phase at 12 h after treated with 100 μg/ml LPS, but the cell number decreased in G1 phase, and increased in S phase after treated with Chinese baicalin. Conclusion：Chinese herb baicalin can significantly promote the proliferation of PDLC. It can make some static cell in G1 phase enter S phrase and improve the growth of human PDLC.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2008年第5期724-727,共4页
Journal of Practical Stomatology
关键词
脂多糖
黄芩苷
细胞培养
牙周膜细胞
流式细胞仪
MTT法
Lipopolysaccharides
Baicalin
Cell culture
Periodontal ligament cells
Flow cytometry
MTT method
作者简介
通讯作者：李会英 0311-85911014
