摘要
体外培养Wister大鼠颅骨成骨细胞,分别置于0Gs、400Gs、620Gs、830Gs、1080Gs不同磁场强度的静磁场中作用24h、48h和72h。用噻唑兰(MTT)法检测成骨细胞的增殖情况,流式细胞仪测定不同磁场强度下细胞所处的周期。结果显示,各组均出现不同程度的促增殖作用,尤以400Gs和620Gs组作用72h时显著;620Gs磁场治疗组S期、G2/M期细胞百分比显著增加。推测一定磁场强度可促使静止态的G1期细胞活跃,向S期转变,细胞增殖加速。
Rat calvarias osteoblasts were cultured and put in the static magnetic field of 0Gs, 400Gs, 620Gs, 830Gs and 1080Gs for 24h, 48h and 72h, respectively. MTT method was used for detecting osteoblast proliferation and flow cytometry for detecting cell cycle. When the magnetic exposure time extended to 48h or 72h, the corresponding OD value of osteoblast with magnetic treatment of 400 Gs or 620 Gs increased obviously with significant difference as compared with 0Gs group (P <0.05), but such proliferative effect was not shown in the 1080 Gs group (P >0.05). After magnetic exposure with intensity of 620 Gs, G0/G1 phase percentage decreased, but S phase and G2/M phase percentage was significantly higher as compared with control. Proliferation index (PI) increased as well and there was significant difference in contrast to control (P <0.05). Within a certain range of magnetic intensity, magnetic field enhances cell growth in a dose-dependent pattern. And static magnetic field can activate static G1 phase into S phase, increase DNA synthesis, and accelerate cell proliferation.
出处
《上海口腔医学》
CAS
CSCD
2004年第5期469-471,共3页
Shanghai Journal of Stomatology
基金
辽宁省教育厅基金(20122170)
