摘要
目的通过不同途径行Sertoli细胞-肝脏联合移植，探讨Sertoli细胞是否可为移植肝提供免疫保护。方法“2步法”分离培养Sertoli细胞，“二袖套管法”行大鼠原位肝移植并以Wistar→SD组合建立排斥反应模型。通过三种途径进行Sertoli细胞-肝脏联合移植。分别观察术后各组症状、体征、肝功能变化、移植肝病理特征等。采用免疫组化、凋亡等技术检测Sertoli细胞功能及作用，探讨其对肝移植急性排斥的影响。结果肝移植急排模型不干预组14只，1只存活超过14d。Sertoli细胞腹腔注射组、阴茎背静脉注射组和供体移植前门静脉注射组分别有5、8、7只存活超过14d。各干预组存活率与对照组比较：后两组差异有显著性（P〈0．05），腹腔注射组差异不显著（P〉0．05）；各干预组之间存活率差异无显著性（P〉0．05）。供肝病理检查显示各干预组排斥反应较对照组轻。免疫组化及凋亡检测发现：肝移植后14d，Sertoli细胞仍存活并表达FasL，Sertoli细胞周围有淋巴细胞集聚及凋亡的淋巴细胞。结论Sertoli细胞对肝移植急性排斥有抑制作用，对供肝有诱导免疫耐受作用，Sertoli细胞通过Fas／FasL途径诱导淋巴细胞凋亡。
Objective To evaluate the function of immunity protection of Sertoli cells post liver transplantation by transferring the cultured Sertoli cells into acute rejection animal model of liver transplantation in rats. Methods The Sertoli cells were isolated and cultured. The animal model of liver transplantation and the acute repulsion animal model of liver transplantation were established in rats. As for co-transplantation of Sertoli cells with liver, we applied three ways. The function of liver, pathological change and signs after surgery were compared among different groups. By using the immunohistochemistry and apoptosis assay technique, the function of Sertoli cells to strive the acute repulsion of liver transplantation was determined. The data were analyzed with SPSS 12.0 by ANOVA and t-test. Results The animal model of Wistar→SD showed the most severe acute rejection. Thirteen rats from the group（n= 14） of animal model of Wistar→SD with no Sertoli cells co-transplantation died within 14 days after liver transplantation. The group （n=14） of injecting the Sertoli cell suspension into celiac cavity had 5 rats living longer than 14 days. The group （n=14） of injecting Sertoli cells suspension intravenously had 8 rats living longer than 14 days. The group （n=14） of injecting the Sertoli cell suspension into portal vein of donor＇s prior to the surgery of liver taking out had 7 rats living longer than 14 days. Pathological examination of liver showed that the groups of co-transplantation had significantly slighter acute rejection than the control group. The results of immunohistochemistry and apoptosis assay showed that Sertoli cells were still alive and secreted the factor of FasL. Around the Sertoli cells, there was assembling of lymphocytes with some apoptotic lymphocytes after 14 days of co-transplantation. Conclusion Sertoli cells can restrain the acute rejection in liver transplantation and induce immune tolerance in donor liver.
出处
《中华肝胆外科杂志》
CAS
CSCD
2007年第7期480-483,共4页
Chinese Journal of Hepatobiliary Surgery
作者简介
通讯地址：510660广州市，广州市天河区广氮新村内暨南大学附属第一医院东圃分院）电子信箱：liulixin@tom．com
