摘要
目的 探讨大鼠原位肝移植死亡的原因及预防措施。方法 对改良法大鼠原位肝移植死亡原因及预防措施进行回顾性总结分析。结果 210只大鼠行原位肝移植,其中19例死亡。引起死亡原因有: 肝上下腔静脉出血5例,肝下下腔静脉狭窄血栓形成2例,左膈下静脉出血2例,胆管梗阻 3 例,门静脉狭窄血栓形成 2 例,感染2例,麻醉过深1例,呼吸衰竭1例,肝脏损伤1例。结论 熟练的显微外科技术、细致的手术操作是预防术后并发症的先决条件; 麻醉的成功控制是手术成功的保障; 受体无肝期的长短是决定动物生存质量的关键; 改善手术条件及合理的抗菌素应用是预防术后感染的必要手段。
Objective To investigate the causes of death in recipient rats after modified orthotopic liver transplantations and the countermeasure.Methods Two hundred and ten orthotopic liver transplantations were performed in Wistar and SD rats weighing 250～300 g through modified Kamada’s cuff technique, and to investigate the causes of death and preventive measures. Results It took about (41±5) min, (13±2) min and (45±5) min for donor operation,donated liver trimming and recipient operation respectively. The anhepatic phase was (20±5) min. The 4 week survival after transplantation was 90.5%. Nineteen rats died during and after transplantation. The causes of death included: bleeding of suprahepatic inferior vena cava ( n =5 ), infrahepatic vena cava thrombosis ( n = 2), biliary obstruction ( n =3 ), portal vein constriction and thrombosis ( n =2 ), liver injury( n =1), bleeding of left subphrenic vein ( n =2 ), infection ( n =2 ), excessively deep anesthesia ( n =1), and respiratory failure ( n =1). Conclusion The sophisticated microsurgical technique and the delicate surgical manipulation are very important in preventing operative complications, and the short anhepatic phase of recipient is the key to animal survival, and proper anesthesia is also important to the success of operations. Improving the condition of operation and reasonable use of antibiotics are necessary in preventing infections.
出处
《中国普外基础与临床杂志》
CAS
2005年第2期120-122,共3页
Chinese Journal of Bases and Clinics In General Surgery
