摘要
目的 :探讨如何预防大鼠原位肝移植术的近期并发症。方法 :在Kamada等用袖套法吻合血管的基础上 ,进行改进 ,供体改经腹主动脉进行肝脏冷灌注 ,肝上下腔静脉用缝合法吻合 ,门静脉和肝下下腔静脉用袖套法吻合 ,胆总管采用单管内支架胆管端端吻合法 ,共施行大鼠原位肝移植术 36 0次 ,供体手术时间为(31.2± 5 )min ,受体手术时间为 (4 5± 5 .5 )min ,无肝期为 (2 0± 2 .5 )min。结果 :术后近期主要并发症有 :出血12例 ,肝下下腔静脉血栓 8例 ,袖套扭转或脱落 4例 ,胆管梗阻 3例。结论 :娴熟的显微外科技术、细致的手术操作是预防术中和术后并发症的先决条件。受体无肝期的长短是决定动物存活的关键。
Objective:To investigate how to prevent the short-term complications after orthotopic liver transplantation in rats.Methods:On the basis of two-cuff technique,the donor liver was perfused through the abdominal aorta with cold perfusate. The anastomosis of the suprahepatic vena cave was sutured end-to-end with 8-0 nylon line,and the continuty of infrahepatic vena cave and portal vein were established by means of cuff method respectively. The bile duct anastomosis was performed by internal stent. Orthotopic liver transplantations were performed in 360 rats. The operation time for donor and recipient were(31.2±5)min and (45±5.5)min,respectively,and anhepatic time was(20±2.5)min.Results:The main postoperative complications included:bleeding in 12 rats,infrahepatic vena cave thrombosis in 8 rats,cuff twisting or dropping in 4 rats,and biliary obstruction in 3 rats.Conclusion:The sophisticated microsurgical technique and the delicate surgical manipulation is the prerequisite of preventing operational complication,and shortening the anhepatic phase of recipient as soon as possible is the key to animal survival.
出处
《肝胆胰外科杂志》
CAS
2003年第4期223-225,共3页
Journal of Hepatopancreatobiliary Surgery
关键词
大鼠
肝移植
并发症
rat
liver transplantation
complications
