摘要
以葡萄糖氧化酶(GOD)和过氧化氢酶(CAT)协同作用提纯商品异麦芽低聚糖,并采用均匀设计法结合单因素试验确定了最佳工艺条件:当温度40℃,pH 4.0,底物质量浓度200 g/L,GOD的用量100 U/g,CAT与GOD的酶活力比为15时,其中的葡萄糖被完全除去,异麦芽低聚糖的纯度由62.78%提高到85.28%.
Commercial isomalto-oligosaccharide was purified by combined enzyme of glucose oxidase （GOD） and catalase（CAT）. The optimal conditions for the reaction were obtained by using uniform design and single factor experiment, which were 40 ℃, pH 4. 0, 200 g/L sugar concentration, 100 U/g GOD concentration and 15 of ratio of GOD to CAT. The glucose was completely eliminated, and the purity of isomalto-oligosaccharide was increased to 85.28% from 62. 78%.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2005年第4期102-105,共4页
Journal of South China Agricultural University
基金
广东省火炬计划资助项目(99M01404H)
关键词
复合酶
提纯
异麦芽低聚糖
combined enzyme
purify
isomaho-oligosaccharide
作者简介
徐小艳（1977-），女，硕士；E-mail：yanzixu-2001@sohu．com
