摘要
目的 探究淋巴细胞免疫治疗(LIT)对小鼠自然流产模型母胎界面T细胞免疫球蛋白黏蛋白-3(Tim-3)和半凝乳素-9(Gal-9)表达水平的影响.方法 构建CBA/J×BALB/c正常妊娠模型及CBA/J×DBA/2自然流产模型,8只正常妊娠模型小鼠无特殊处理纳入正常妊娠组,8只仅行麻醉的正常妊娠小鼠纳入正常妊娠麻醉干预组,8只自然流产模型小鼠合笼前行LIT治疗纳入自然流产LIT干预组,8只自然流产模型小鼠合笼前注射等量生理盐水纳入自然流产无干预组.于妊娠第12~14天观察各组小鼠胎盘丢失率,采用蛋白印记杂交测定各组小鼠Tim-3/Gal-9蛋白表达水平,采用定量PCR测定Tim-3和Gal-9 mRNA表达水平.结果 自然流产无干预组孕鼠胚胎丢失率显著高于自然流产干预组、正常组及正常妊娠麻醉干预组(P<0.05);与正常妊娠模型小鼠比较,模型组无干预小鼠蜕膜Tim-3蛋白及mRNA表达显著升高(P<0.05),Gal-9蛋白及mRNA表达显著下降(P<0.05);LIT干预组小鼠蜕膜Tim-3/Gal-9蛋白及mRNA表达水平与正常妊娠小组比较差异无统计学意义(P>0.05).结论 淋巴细胞免疫治疗可有效降低自然流产模型小鼠的胚胎丢失率,其机制可能与其对Tim-3/Gal-9表达水平的调节有关.
Objective To investigate the effect of lymphocyte immunotherapy(LIT)on the expression level of T-cell immunoglobulin and muein-3(Tim-3)and galetin-9(Gal-9)in maternal-fetal interface of spontaneous abortion mice models.Methods The CBA/J×BALB/c normal pregnancy model and CBA/J×DBA/2 spontaneous abortion model were established.Eight normal pregnant mice models without special treatment were included in the normal pregnancy group.Eight normal pregnant mice treated with anesthesia alone were included in the normal pregnancy+anesthesia intervention group.Eight mice models with spontaneous abortion treated with LIT before being caged were included in the spontaneous abortion+LIT intervention group.Eight mice models with spontaneous abortion injected with the same volume of normal saline before being caged were included in the spontaneous abortion+non-intervention group.The placental loss rate in each group was determined during 12-14 d of pregnancy.The protein expression level of Tim-3/Gal-9 in each group was determined by Western blotting,and the mRNA expression level of Tim-3/Gal-9 was determined by quantitative PCR.Results The embryo loss rate in the spontaneous abortion+non-intervention group was significantly higher than that in the spontaneous abortion+intervention group,normal pregnancy group and normal pregnancy+anesthesia intervention group(P<0.05).Compared with the normal pregnancy group,the protein and mRNA expression levels of Tim-3 significantly increased in the decidua of the non-intervention group(P<0.05),whereas the protein and mRNA expression levels of Gal-9 significantly decreased(P<0.05).There were no statistically significant differences in the protein and mRNA expression levels of Tim-3 and Gal-9 in the decidua between the LIT intervention group and the normal pregnancy group(P>0.05).Conclusion LIT may effectively decrease the embryo loss rate in spontaneous abortion mice models,and its mechanism may be related to the regulation of Tim-3/Gal-9 expression level.
作者
李怡梅
杨小风
王雅莉
Li Yimei;Yang Xiaofeng;Wang Yali(Department of Obstetrics and Gynecology,Zhengzhou Central Hospital Affiliated to Zhengzhou University,Zhengzhou,Henan 450007,China)
出处
《中华生物医学工程杂志》
CAS
2018年第6期402-406,共5页
Chinese Journal of Biomedical Engineering
基金
郑州市中心医院科研项目(18B310036).
关键词
免疫治疗
自然流产
小鼠
T细胞
妊娠
Immunotherapy
Spontaneous abortion
Mice
T cells
Pregnancy
作者简介
通信作者:杨小风,Email:yangxiaofeng1932@126.com
