摘要
目的探讨宫颈癌细胞表达T-cell immunoglobulin and mucin domain containing 3 protein(Tim-3)与宫颈癌恶性进展的关系。方法用反转录PCR(RT-PCR)、Western blot和共聚焦显微镜检测宫颈癌细胞系HeLa和SiHa中Tim-3基因和蛋白的表达及其胞内定位;用免疫组织化学法检测43例宫颈癌组织中Tim-3的表达,并分析其与临床分期、组织分型、转移等相关性。结果证实HeLa和SiHa两个宫颈癌细胞系均表达Tim-3,在前者Tim-3分布于胞质,而在后者则分布于核内及其周围。在宫颈癌患者病理标本中可见癌巢和肿瘤血管高表达Tim-3,且Tim-3表达强度与与临床分期、组织分型和转移相关。结论宫颈癌细胞及血管内皮细胞均高表达Tim-3,且Tim-3表达强度与宫颈癌恶性进展及转移高度相关。
Objective To confirm the relationship between Tim-3（T-cell immunoglobulin and mucin domain containing 3 protein） expression in cervical cancer and the progression of the tumor.Methods RT-PCR,Western blot and confocal microscopy were used to detect the expression of Tim-3 in cervical cancer cell lines,HeLa and SiHa,at both gene and protein levels and its intracellular distribution.Meanwhile,specimens from 43 patients with cervical cancer were detected the expression of Tim-3 by immunohistochemistry.We analysed the relation of Tim-3 expression to clinical stage,tissue typing,metastasis.Results Tim-3 was expressed highly in the HeLa and SiHa cells.Moreover,according to the statistics results,Tim-3 expressed highly in the cancer cell,relative to the increased degree of malignancy of cervical cancer.Conclusion Tim-3 is expressed highly in cervical cancer cell and vessel endothelial cell,moreover it is relative to the increased degree of malignancy of cervical cancer.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2012年第11期1353-1356,共4页
Cancer Research on Prevention and Treatment
基金
国家自然科学基金青年科学基金资助项目(81001049)
作者简介
周晓曦（1984-），女，博士在读，主要从事肿瘤免疫方面的研究
通信作者：曹阳E-mail：caoyangemma@163．com
