摘要
为获得抗芜菁花叶病毒病、耐抽薹的大白菜植株,进行了双价反义植物表达载体的构建和遗传转化研究。在利用TuMV-Nib、LFY基因,构建成反义双价植物表达载体,转化农杆菌LBA4404,获得工程菌株的基础上,采用农杆菌介导的真空渗入法转化大白菜4个高代自交系材料,获得了经PCR检测和TuMV感染鉴定的16株转基因植株。经田间鉴定有7株表现为抗病毒和耐抽薹。该结果可为抗病毒病、耐抽薹的转基因大白菜提供种质材料。
In order to obtain the cabbage plant resistant to turnip mosaic virus disease and bolting, the construction and genetic transformation of bivalent antisense plant expression vectors were studied. Using TuMV-Nib and LFY genes, an antisense bivalent plant expression vector was constructed and transformed into Agrobacterium LBA4404. Based on the engineering strain, Agrobacterium-mediated vacuum infiltration method was used to transform 4 Chinese cabbage inbred lines, and 16 transgenic plants identified by PCR and TuMV infection were obtained. Seven strains were identified as resistant to TuMV virus and bolting in the field. It provides germplasm materials for transgenic Chinese cabbage resistant to antiviral diseases and bolting.
作者
佟容
乔宏宇
王智博
吴春燕
陈姗姗
孙雨晴
于占东
TONG Rong;QIAO Hongyu;WANG Zhibo;WU Chunyan;CHEN Shanshan;SUN Yuqing;YUZhandong(College of Horticulture,Jilin Agricultural University,Changchun 130118,China)
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2020年第5期533-537,共5页
Journal of Jilin Agricultural University
基金
吉林省教育厅“十三五”科技项目(JJKH20170314KJ)
关键词
大白菜
双价反义载体
无选择标记转基因
遗传转化
Chinese cabbage
bivalent antisense vector
non-selective marker transgene
genetic transformation
作者简介
佟容,女,在读硕士,研究方向:蔬菜栽培生理与生物技术;通信作者:于占东
