摘要
以中国大白菜花蕾为材料,通过RT-PCR方法,参照GeneBank公布的序列克隆了Leafy(简称LFY)基 因片段,测序分析结果表明,该基因与国外已经报道的序列具有94％的同源性。将此序列以相反方向插入到 植物表达载体pROK2 35S启动子之间,并在2反向重复片段之间插入小麦乙酰辅酶A羧化酶的第20内含子, 从而构建了dsRNA抑制双元载体,该载体的构建为通过农杆菌介导转化大白菜进而解决早春和反季栽培大 白莱的先期抽薹问题创造了条件。
Through RT - PCR and according to the sequence on Gene Bank, the LFY gene was cloned from Chinese cabbage bud. Sequence analysis showed that it was of 94% homology with the gene reported abroad. When the inverse gene fragment was inserted into 35S promoter of pROK2 plant expression vector and into the 20th intron of AS - COA carboxylase of wheat, dsRNA inhibitor vector was constructed.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2004年第6期615-619,共5页
Journal of Jilin Agricultural University
基金
山东省蔬菜良种产业化开发项目
