摘要
目的网络药理学方法,研究乳香-没药药对在抗溃疡、抗炎、抗肿瘤和炎症性肠病和镇痛的治疗过程中的共性作用机制。方法借助中药系统药理学分析平台(TCMSP)检索乳香-没药药对的化学成分和作用靶点,通过人类基因数据库(Genecards)和在线人类孟德尔遗传数据库(OMIM)等多个数据库筛选上述5种疾病相关靶点。利用Cytoscape3.7.1软件构建"药物-靶点-疾病"交互网络图。运用蛋白相互作用(PPI)筛选核心靶点;通过生物学信息注释数据库(DAVID)做基因本体(GO)功能分析和基因组百科全书(KEGG)通路分析。结果乳香-没药药对共包含403个化合物,247个活性靶点,与五种疾病基因交集获得79个高频基因,通过构建"药物-靶点-疾病"网络及对网络进行分析,共获得20个关键靶标;在对GO富集分析时,获得8个高频共性相关条目,主要病毒感染和肿瘤信号通路等;在对靶标进行KEGG通路分析时,共获得6条高频相关的通路,其中病毒感染和肿瘤信号通路为主要通路。结论本研究结果初步验证了乳香-没药药对在抗溃疡、抗炎、抗肿瘤、炎症性肠病和镇痛这五种疾病治疗的作用机制以及高频共性靶点、基因、信号通路等,为进一步阐述乳香-没药药对作用机制奠定了良好的理论基础。
Objective To explore the mechanism of network pharmacology of frankincense and myrrh in anti-ulcer,anti-inflammation,anti-cancer,inflammatory bowel disease and analgesic treatment.Methods Frankincense and myrrh pair active ingredients`relevant chemical structure and corresponding drug targets were obtained by the Traditional Chinese Medicine Systems Pharmacology Database(TCMSP);Five diseases related targets were screened by Online Mendelian Inheritance in Man(OMIM)and The Human Gene Database(GeneCards);The"drug-gene-disease"networks were constructed by Cytoscape 3.7.1;The core targets were screened by Protein-Protein Interaction Networks(PPI);Gene Ontology(GO)function enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment were analyzed by the Database for Annotation,Visualization and Integrated Discovery(DAVID).Results We concluded that the volatile oil components were the main chemical component by"herb-gene-drug"networks,and 20 high frequency common targets,8 high frequency common entries and 6 KEGG high frequency common path ways were obtained at the same time.Conclusion The common mechanisms of frankincense and myrrh pair in anti-ulcer,antiinflammation,anti-cancer,inflammatory bowel disease,analgesia,common high frequency targets,genes and KEGG pathways discovered by network pharmacological methods provide theoretical basis experimental date for further research and clinical application of frankincense active ingredients.
作者
周宜
邓蓝冰
周游宇
孟祥龙
Zhou Yi;Deng Lanbing;Zhou Youyu;Meng Xianglong(The Second Affiliated Hospital of Hunan University of Traditional Chinese Medicine,Changsha,410005,China;Shanxi University of Traditional Chinese Medicine,College of Chinese Medicine and Food Engineer⁃ing,Jinzhong,030619,China)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2020年第7期2338-2347,共10页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
湖南省中医药科研计划一般项目(201997)
油胶树脂类中药(乳香、没药)热解特性与其炮制加工相关性研究,负责人:周宜
山西中医药大学科技创新能力培育计划(2019PY-154):乳香和没药炮制工艺及“炮制工艺-挥发性成分-生肌”关联性研究
负责人:孟祥龙
关键词
网络药理学
乳香-没药药对
抗溃疡
抗炎
抗肿瘤
炎症性肠病
镇痛
药效机制
Network pharmacology
Frankincense-Myrrh herbal pair
Anti-ulcer
Anti-inflammatory
Anti-tumor
Inflammatory bowel disease
Analgesia
Pharmacodynamic mechanism
作者简介
通讯作者:孟祥龙,硕士,实验师,主要研究方向:中药炮制及药效机制研究。
