摘要
目的通过测定黄芩粉末的色度及有效成分黄芩苷的含量,将代表颜色与代表质量的内在含量指标值相关联,探讨黄芩药材化学成分与色度之间的相关性,为中药黄芩的等级标准及质量评价提供理论依据。方法利用色差仪对黄芩粉末进行测定,以2015年版《中华人民共和国药典》中黄芩含量测定项下方法,采用高效液相色谱(HPLC)进行测定,通过SPSS 21.0软件进行统计分析,同时结合药材内部物质成分含量测定结果,对二者间相关性进行研究分析。结果黄芩中黄芩苷含量与a*(代表颜色红绿色度)、b*(代表颜色黄蓝色度)存在极显著正相关关系(P<0.01),表明在一定程度上a*、b*值越大,黄芩苷含量越高;而黄芩苷含量与L*(代表颜色明暗)存在极显著负相关关系(P<0.01),表明在一定程度上,L*值越小,黄芩苷含量越高。结论黄芩外观颜色与有效成分黄芩苷含量相关,且颜色深棕黄色的黄芩,黄芩苷含量较高,质量相对较好。通过色差仪的测定可初步快速判断黄芩药材的有效成分含量,为黄芩药材等级划分及制订药材的质量标准提供参考依据,并且在其生产及商品流通等方面也具有一定的指导意义。
Objective Through the determination of the color of Scutellaria baicalensis powder and the content of baicalin,the intrinsic content index value of representative color and representative quality was correlated,and the correlation between chemical composition and color of Scutellaria baicalensis was discussed,which provided theoretical basis for the grade standard and quality evaluation of Scutellaria baicalensis.Methods Scutellaria baicalensis powder was determined by chromatograph,and the content of Scutellaria baicalensis in Chinese Pharmacopoeia 2015 edition was determined by HPLC.Statistical analysis was carried out by SPSS 21.0 software.At the same time,the correlation between the two was studied and analyzed by combining the results of the content determination of the internal components of medicinal materials.Results The content of Baicalin in Scutellaria baicalensis was positively correlated with a~*(representing red-green color)and b~*(representing yellow-blue color)to a certain extent(P<0.01),indicating that,to some extent,the higher the value of a~*and b~*,the higher the content of baicalin,while the content of baicalin was negatively correlated with L~*(representing light and dark color)(P<0.01),indicating that to a certain extent,the smaller the value of L~*,the higher the content of baicalin.Conclusion The appearance color of Scutellaria baicalensis is correlated with the content of baicalin,and the content of deep brown yellow Scutellaria baicalensis and baicalin is relatively high and the quality is relatively good.The content of active ingredients in Scutellaria baicalensis can be determined by colorimeter,which can provide reference for grading and quality standards of Scutellaria baicalensis.It also has a certain guiding significance in production and commodity circulation of Scutellaria baicalensis.
作者
武艳雪
翁丽丽
姜雨昕
宿莹
侯晓琳
Wu Yanxue;Weng Lili;Jiang Yuxin;Su Ying;Hou Xiaolin(Changchun University of Traditional Chinese Medicine,Changchun 130117,China)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2020年第8期2839-2844,共6页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家中医药管理局重大课题(ZYBZH-Y-JL-25):甘草等七种中药饮片标准化建设,负责人:韩英彪
关键词
黄芩
黄芩苷
色度
相关性
Scutellaria baicalensis
Baicalin
Chroma
Correlation
作者简介
通讯作者:侯晓琳,实验师,主要研究方向:中药品质评价与资源开发。
