摘要
丛枝菌根真菌(AMF)是一种存在于生态系统中重要的土壤微生物,广泛应用于植物的防旱、抗旱。本研究选用紫花苜蓿(Medicago sativa)为试验材料,分别研究了接种丛枝菌根真菌、磷和水分对其生物量、株高、脯氨酸和丙二醛含量的影响。结果表明,磷浓度变化对紫花苜蓿生长发育状况影响不显著,而接种AMF后可大幅度改善紫花苜蓿的抗旱性能。其中,水分充足(即田间持水量75%~80%)条件下紫花苜蓿生长状况最好;随着水分降低,紫花苜蓿生物量与株高显著下降,脯氨酸、丙二醛含量增加,酶活性下降。接种AMF对紫花苜蓿的生长状况以及相关生理生化指标均有显著改善作用。综合接种AMF和磷处理结果显示,施加0.25 mmol·L-1KH2PO4Hogland营养液并接种AMF的试验组抗旱效果最好,说明在干旱胁迫下,一定施磷水平下接种AMF可有效地提高紫花苜蓿的抗旱性。
Arbuscular mycorrhizal fungi（ AMF） are soil microorganisms that play important roles in the ecosystem.AMF have been widely used for drought resistance in plants. The alfalfa cultivar WL 168 was used to examine the effect of AMF and P on plant biomass and plant growth,proline,MDA,and other physiological and biochemical properties under drought stress. The results demonstrated that the change in P concentration had no significant effect on the growth of alfalfa,but the drought resistance of alfalfa could be significantly improved after the use of AMF. Alfalfa growth was best in the presence of sufficient water content（ 75% ~ 80%）. As water content decreased,the biomass and plant height of alfalfa decreased significantly,proline and malondialdehyde content increased,and SOD activity decreased. Inoculation with AMF on alfalfa growth status and related physiological and biochemical indicators were significantly improved. The results of AMF and P treatment showed that the drought resistance of Hogland（ KH2PO4： 0. 25 mmol·L-1） nutrient solution and AMF was the best,which indicated that,under drought stress,AMF applied at the same time to use a certain level of P can effectively improve the drought resistance of alfalfa.
出处
《草业科学》
CAS
CSCD
2018年第1期115-122,共8页
Pratacultural Science
基金
国家牧草产业技术体系(CARS-35)
关键词
紫花苜蓿
磷
丛枝菌根真菌
水分胁迫
菌根侵染率
抗旱性能
alfalfa
P
arbuscular mycorrhizal fungi
water stress
mycorrhiza infection rate
drought resistance
作者简介
林子然（1992-）,女,黑龙江牡丹江人,在读硕士生,研究方向为草地资源与生态.E-mail：429580036@qq.com;通信作者：张英俊（1971-）,男,内蒙古四子王旗人,教授,博导,博士,研究方向为草地管理与生态.EGmail：zhangyj@cau.edu.cn
