摘要
为探明干旱胁迫下印度梨形孢提高烤烟抗旱能力的机理,以云烟87为试验材料,自然干旱7d,分析了生理生化指标及干旱相关基因表达的变化。结果表明,印度梨形孢能够定殖于烤烟根部,促进烤烟地上和地下部分生长,且干旱胁迫后叶片褪绿不明显,干旱症状轻微。在干旱胁迫下,与对照相比,接种印度梨形孢烤烟叶片中叶绿素a、叶绿素b和总叶绿素含量降低缓慢,可溶性蛋白和Pro含量显著升高,MDA含量降低。随干旱处理时间延长,接种印度梨形孢烤烟叶片SOD和POD活性呈现先升高后下降的趋势,干旱相关基因NTNAC1和NAC4的表达上调,且显著高于对照植株。文中研究初步明确了印度梨形孢提高烤烟抗旱能力的机理,为深入研究印度梨形孢的抗逆性和烤烟在干旱地区的种植生产提供了理论基础。
In order to explore the mechanism of Piriformospora indica enhancing drought resistance of flue-cured tobacco under drought stress,Yunyan 87 was used as the experimental material,and the changes of physiological and biochemical indexes and the expression of related drought resistance genes were analyzed under natural drought for 7 days.The results showed that P.indica could colonize the root of flue-cured tobacco and promote the growth of above-ground and underground parts of flue-cured tobacco,and chlorosis of leaves and symptoms of their drought were not obvious.Under drought stress,the content of chlorophyll-a,chlorophyll-b and total chlorophyll reduced slowly,soluble protein and Pro increased significantly,and contents of MDA reduced in flue-cured tobacco inoculated with P.indica.With the extension of drought treatment time,the SOD and POD activities in leaves increased firstly and then decreased,the expression of drought-related gene NtNAC1 and NAC4 increased,and they were significantly higher than those of the control plants.This study preliminarily clarified the mechanism in P.indica enhancing drought resistance of flue-cured tobacco,and provided a theoretical basis for further research on the stress resistance of P.indica inoculation and the production of flue-cured tobacco in arid areas.
作者
李震
邵晨阳
田仁进
罗富方
韦朝贵
许本波
孙文秀
LI Zhen;SHAO Chenyang;TIAN Renjin;LUO Fufang;WEI Chaogui;XU Benbo;SUNWenxiu(Guizhou Qianxinan Tobacco Company,Xingyi 562400;College of Life Sciences,Yangtze University,Jingzhou 434025,China)
出处
《干旱区资源与环境》
CSSCI
CSCD
北大核心
2023年第3期143-149,共7页
Journal of Arid Land Resources and Environment
基金
贵州省烟草公司黔西南州公司科技项目(202104)
湖北省教育厅科学研究计划重点项目(D20181302)资助
关键词
印度梨形孢
烤烟
干旱胁迫
抗旱能力
Piriformospora indica
flue-cured tobacco
drought stress
drought resistance
作者简介
李震(1983-),男,汉族,湖北邯郸人,硕士,农艺师,主要从事植物逆境生理方面的研究。E-mail:zhenli56@163.com;通讯作者:孙文秀(1979-),女,汉族,山东潍坊人,副教授,硕士生导师,主要从事微生物资源开发与利用研究。E-mail:wenxiusun@163.com
