摘要
本项研究归纳出三种DNA指纹分析方法 ,即特征谱带法、引物组合法和核心引物组合法 ,并比较了这三种方法在玉米品种纯度及真伪鉴定中的应用价值。在玉米基因组上均匀选取 6 2对SSR引物对 6 0个玉米自交系进行分析。 6 2对SSR引物共检测到 2 38个等位基因变异 ,平均每个位点的等位基因数4 0 8个 ,平均多态性信息量 (PIC) 0 6 12 ,平均标记索引系数 (MI) 2 5 8,评估引物多态性的三个指标并不完全一致。三种方法的比较研究表明 ,特征谱带法仅在固定的材料范围内有效 ,当扩大范围后 ,原来具有特征带的样品往往不再具有特征带 ,且需要筛选大量的引物 ,效率很低 ;引物组合法通过不同引物的有限组合 ,完全可以区分全部材料 ,避免了筛选大量引物的工作 ;核心引物组合法是引物组合法的扩展 ,不同实验室通过使用固定的一套核心引物组合 ,其指纹图谱可以相互比较和整合 ,为玉米DNA指纹图谱分析的标准化奠定基础。
Three DNA fingerprint analyzing methods including distinct band method, primer combination method and core primer combination method had been generalized in this research and compared their usefulness in maize cultivars' identification. A set of 60 elite maize (Zea mays L.) inbred lines were assayed with 62 pairs of SSR primers. 238 alleles were found with average alleles 4.08,average polymorphism information content (PIC) 0.612 and average marker indices (MI) 2.58 per locus. The three index evaluating primer polymorphism (average alleles, PIC, MI) were not completely coherent. It was manifested from three methods' comparison that distinct band method was useful only in fixed materials. When the materials enlarged, samples originally with distinct band often no longer had distinct band; on the other hand, its efficiency was low due to need screening a lot of primers. Primer combination method could discriminate all materials by different primers' limited combinations, avoided of a lot of primer-screening. Core primer combination was an extension of primer combination method, data from different laboratories could be compared and integrated by using a set of fixed core primer combination and it would lead to standardization of maize DNA fingerprint analysis.
出处
《分子植物育种》
CAS
CSCD
2003年第5期655-661,共7页
Molecular Plant Breeding
基金
北京市重点项目(H030730010690)资助。
作者简介
赵久然,通讯作者,jiuran@263.net
