摘要
花椒系芸香科植物花椒Zanthoxylum bungeanum或青椒Z.schinifolium的干燥成熟果皮,为著名的川产道地药材,尤以四川汉源、茂县、九龙等地区栽培的"大红袍"享誉海内外,但有关川产花椒"道地性"的遗传学基础研究较为薄弱。该文将四川省(汉源县、九龙县、理县、茂县)与甘肃武都、陕西凤县共6个产地的花椒进行转录组分析,Trinity组装后得到177616条unigene,通过与KEGG,NR,SwissProt,Trembl,KOG/COG,GO,Pfam数据库比对,最终获得106644条有注释信息的unigene;川产与其他产区的花椒果实中共4574个差异基因,3740个基因上调表达,834个基因下调表达,其中,27个上调基因与萜类化合物相关,8个上调基因与异喹啉类生物碱生物合成相关,不同产地花椒样本间差异表达基因的富集比较显示不同产地样本间存在明显差异;KEGG通路分析表明,川产与其他产区的花椒果实及叶片中的差异信号都主要集中在植物病原交互、植物激素信号转导、苯丙烷代谢等通路,而异黄酮合成通路、甜菜素合成通路在果实和叶片中有明显差异。该研究为后续比较不同产地的花椒品质差异与基因表达差异间的关系奠定了基础。
"Huajiao"is dried ripe fruit peel of Zanthoxylum bungeanum or Z.schinifolium,is konwn as geoherbs,especially the"Dahongpao"cultivated in Hanyuan,Maoxian and Jiulong of Sichuan province.However,the genetic basis of Dao-di"Huajiao"is virtually unknown.The transcriptome of the fruit and leaf from Sichuan(Hanyuan,Jiulong,Lixian,Maoxian),Gansu(Wudu)province and Shaanxi(Fengxian)province was sequenced.Trinity de novo assembling resulted in a total of 177616 unigenes.Through the KEGG,NR,SwissProt,Trembl,KOG/COG,GO,Pfam database comparision 106644 annotated Unigene finally,4574 deferentially expressed genes were found in fruit between Sichuan and other provinces,including 3740 up-regulated genes and 834 down-regulated genes.Among the up-regulated genes,27 up-regulated genes were raleted to terpenoids,and 8 up-regulated genes were related to isoquinoline alkaloid bio-synthesis.Furthermore,it was also showed remarkable differences in groups which enrichment ratio of the diffe-rent expressed gene compared.The different expressed genes were annotated by the KEGG database into plant-pathogen interaction,plant hormone signal transduction and phenylpropanoid biosynthesis in fruit and leaf,but isoflavonoid bio-synthesis and betaine bio-synthesis were significantly different in fruit and leaf.The study laid a certain reference basis for comparison of quality and different expressed gene of Z.bungeanum from different groups.
作者
华桦
严志祥
田韦韦
刘俐
孙洪兵
周先建
鄢良春
赵军宁
HUA Hua;YAN Zhi-xiang;TIAN Wei-wei;LIU Li;SUN Hong-bing;ZHOU Xian-jian;YAN Liang-chun;ZHAO Jun-ning(Institute of Translational Pharmacology and Clinical Application,Sichuan Center for Translational Medicine of Traditional Chinese Medicine,Sichuan Engineering Technology Research Center of Genuine Regional Drug,Biological Assay Key Laboratory of State Administration of Traditional Chinese Medicine for Traditional Chinese Medicine Quality,Sichuan Provincial Key Laboratory of Quality Evaluation and New Drug Creation of Traditional Chinese Medicine,Laboratory for Rapid Detection of Toxicity of Traditional Chinese Medicine in Sichuan Provincial Administration of Chinese Medicine,Sichuan Academy of Chinese Medicine Sciences,Chengdu 610041,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2020年第4期732-738,共7页
China Journal of Chinese Materia Medica
基金
四川省中医药管理局花椒专项(2018HJZX002)
国家“重大新药创制”科技重大专项(2018ZX09201018-029)
四川省科技厅重点研发项目(2018SZ0056)
2017年中医药公共卫生服务补助专项(财社[2017]66号)
2018年中医药公共卫生服务补助专项(财社[2018]43号).
关键词
川产道地药材
花椒
转录组
品质
Sichuan Dao-di herbs
Zanthoxylum bungeanum
transcriptome
quality
作者简介
通讯作者:赵军宁,研究员,博士生导师,E-mail:zarmy@189.cn
