摘要
在碱性条件下 ,N_溴代琥珀酰亚胺(NBS)氧化盐酸苯乙双胍 ,体系中荧光素作为能量转移剂 ,在十六烷基三甲基溴化铵(CTAB)的增敏作用下产生化学发光 ;基于此 ,结合流动注射技术 ,建立了测定盐酸苯乙双胍的化学发光分析法 ,详细研究了影响化学发光强度的因素 ,并探讨了化学发光可能的机理 ;化学发光信号的增加值ΔI与盐酸苯乙双胍的质量浓度在0.01～30mg/L范围内呈线性关系 ,方法的检出限(3σ)为0.0034mg/L ,对0.03mg/L的盐酸苯乙双胍进行了11次平行测定 ,相对标准偏差为0.44 % ;将本法用于盐酸苯乙双胍片剂中盐酸苯乙双胍的定量分析 ,结果令人满意。
A novel chemiluminescence(CL) system for the determination of phenformin hydrochloride coupled with flow injection analysis was proposed. It was based on the chemiluminescence emitted during the oxidation of phenformin hydrochloride by N-bromosuccinimide(NBS) in alkaline medium using fluorescein as energy transfer. This system was enhanced by CTAB. The effects of various factors on the CL systems were investigated. The possible mechanism of the proposed method was also studied preliminarily. The linear range of the calibration curve was 0.01～30 mg/L with a detection limit of 0.003 4 mg/L(3σ). The RSD was 0.44% at 0.03 mg/L level(n=11). The proposed method was successfully used for the determination of phenformin hydrochloride in tablet.
出处
《分析测试学报》
CAS
CSCD
北大核心
2004年第2期20-22,26,共4页
Journal of Instrumental Analysis
基金
重庆市教委资助科研项目(渝教科[2001]12号
序号101)
