摘要
目的:建立一种测定法莫替丁含量的化学发光新方法。方法:在碱性条件下,N-溴代琥珀酰亚胺氧化法莫替丁,其反应的能量转移给共存的荧光素物质罗丹明B,罗丹明B因此受激发而产生化学发光,十六烷基三甲基溴化铵对此化学发光体系有强烈的增敏作用;反应物混合后0.5s可获发光强度最大值。据此结合流动注射技术,建立了测定法莫替丁的流动注射能量转移化学发光分析新方法。结果:化学发光信号的增加值ΔI与法莫替丁的质量浓度在1.0×10-4～1.0×10-2mg.mL-1范围内呈线性关系;检出限为5.0×10-5mg.mL-1;对5.0×10-4mg.mL-1的法莫替丁进行了11次平行测定,RSD为2.1%。并且片剂中的共存组分和药物中常用的赋形剂不干扰样品测定。结论:本法操作简单、快速,适合于法莫替丁药物的质量控制。
Objective： To establish a rapid method for determination of famotidine concentration. Method： In alkaline medium,famotidine is oxidized by N- bmmosuceinimide to release energy, which transfers to the coexisting fluorescence substance Rhodamine B. Therefore,Rhodamine B is excited to generate chemiluminescence（CL） in the presence of cetyltrimethyl ammonium bromide as an enhancer. CL assay system is combined with flow injection technique,the maximum valume of CL intensity can be obtained within 0.5 s after mixing of reacting substances. Results：The enhancement value（△I） of CL intensity has good linear relationship with concentration of famotidine in the range of 1.0×10^-4 - 1.0 ×10^-2mg · mL^-1. The detection limit is 5.0 ×10^-5 mg· mL^-1 with completing measurement once within 10 s. The RSD is less than 2. 1% for the determination of 5. 0 ×10^-4mg·mL^-1 famotidine （n = 11 ）. Coexisting substances in tablets and vehicle in commonly used drug do not interfere in the determination. Conclusion：This method is convenient,rapid,accurate and may be considered for the quality control of famotidine.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2009年第1期113-117,共5页
Chinese Journal of Pharmaceutical Analysis
关键词
流动注射
能量转移
化学发光
法莫替丁
flow injection
energy transfer
chemiluminescence （CL）
famotidine
作者简介
通讯作者Tel：（0791）3950346；E—mail；nucaw0104@sina．com
