摘要
目的 :评价血清胱抑素C(CystatinC)在早期肾功能损害诊断中的临床意义。方法 :应用ELISA法测定 84例各种肾脏病患者的血清胱抑素C浓度 ,同时测定血清肌酐 (Scr,Jaffe法 )及肌酐清除率 (Ccr) ,以放射性核素99m Tc -DTPA测得的GFR作为诊断评价的金指标 ,比较CystatinC、Scr、Ccr与GFR的相关性 ,采用受试者工作特征曲线下面积 (AUCROC)和似然比 (LR)评价CystatinC的可靠性。 结果 :血清CystatinC、Scr、Ccr与GFR均呈显著相关(P <0 .0 1) ,且以CystatinC与GFR的相关程度最密切 ;上述三个指标AUCROC 分别为 0 .989、0 .92 6、0 .94 2 ,且CystatinC与Scr之间具有显著统计学意义 (P <0 .0 5 ) ,而与Ccr间无统计学差异 (P >0 .0 5 ) ,LR以血清CystatinC为最佳。结论 :血清CystatinC是一个更为准确、可靠的反映肾小球滤过功能的指标 。
Objective:To evaluate the applicability of serum cystatin C in patients with renal impairment.Methods:Serum samples were abtained from 84 cases with renal diseases,Serum cystatin C concentration was determined by enzyme linked immunosorbent assay(ELISA),Serum creatinine(Scr) by Jaffe method and Creatinine clearance rate(Ccr) were measured,glomerular filtration rate(GFR) by 99mTc-DTPA clearance was considered to be a Gold Standard,the correlation between GFR and the above three parameters were compared, the area under the Receiver Operating Charateristic curve(AUC ROC)and the likelihood ratio(LR)were used for evaluating the reliability of cystatin C.Results:The significant correlation were found between GFR and cystatin C、Scr、Ccr,and that of between GFR and cystatin C was the most strongest. The AUC ROCfor cystatin C、 Scr、Ccr was 0.989、0.926、0.942 respectively,and there was significant statistical differentce between cystatin C and Scr(P<0.05),but no statistical difference between cystatin C and Ccr(P>0.05).LR of serum cystatin C was the best among the above three parameters.Conclusion:Serum cystatin C determination is an accuate and reliable parameter of GFR in patients with impaired renal function and there is guidance purpose for diagnosing early renal impairment.
出处
《中国中西医结合肾病杂志》
2004年第2期88-91,共4页
Chinese Journal of Integrated Traditional and Western Nephrology
