摘要
本研究旨在了解美丽硬仆骨舌鱼(Scleropages formosus)基因组微卫星分布规律。采用生物信息学方法对国际濒危物种美丽硬仆骨舌鱼全基因组范围内的微卫星数量及分布规律进行了分析。结果显示:1~6个微卫星重复类型的微卫星序列354239个,总长度为6139487 bp。微卫星单碱基、二碱基、三碱基、四碱基和五碱基重复单元中数量最多的分别为A、AC、AAT、ATCC、AAAAT。25条染色体中,6号染色体所含微卫星数目最多(28662个),22号染色体所含微卫星数目最少(6929个),微卫星数量与其所在染色体DNA序列长度呈线性相关(r=0.999)。22号染色体微卫星出现频率最高(576.35个/Mb),3号染色体微卫星出现的频率最低(469.65个/Mb),结果显示,微卫星频率与其所在染色体DNA序列长度并不相关。本研究为进一步通过微卫星分子标记开展美丽硬仆骨舌鱼亲缘关系鉴定和遗传多样性分析、微卫星标记在硬仆骨舌鱼科的通用性分析奠定了基础。
This study focuses on the distribution of microsatellite in the genome of golden arowana(Scleropages formosus).The quantity and distribution of whole-genome microsatellites of the international endangered species,golden arowana,were analyzed based on bioinformatics methods.The results showed that there were354239 microsatellite sequences with 1-6 microsatellite repetitions,and the overall length was 6139487 bp.The preponderance of mononucleotide,dinucleotide,trinucleotide,tetranucleotide and pentanucleotide were A,AC,AAT,ATCC and AAAAT respectively.Among the 25 chromosomes,chromosome 6 contained the largest number of microsatellites(28662),and the chromosome 22 had the least(6929).Linear correlation(r=0.999)was observed between the number of microsatellites and the length of DNA sequences they located in.However,chromosome 22 had the highest frequency of microsatellite occurrence(576.35/Mb)and chromosome3 had the lowest(469.65/Mb).The result showed that the frequency of microsatellite was not related to the length of the chromosome DNA sequence in which it was located.The research provides a basis for the further study on genetic relationship and genetic diversity in golden arowana,and the transferability of the microsatellite markers.
作者
段永楠
刘奕
胡隐昌
刘超
宋红梅
汪学杰
孙金辉
牟希东
Duan Yongnan;Liu Yi;Hu Yinchang;Liu Chao;Song Hongmei;Wang Xuejie;Sun Jinhui;Mu Xidong(Tianjin Agricultural University,Tianjin300191;Key Laboratory of Recreational Fisheries,Ministry of Agriculture and Rural Affairs,Guangdong Modern Leisure Fisheries Engineering Technology Center,Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou510380)
出处
《中国农学通报》
2019年第23期152-158,共7页
Chinese Agricultural Science Bulletin
基金
“一带一路”沿线国家热带农业科技创新合作项目“热带国家水产养殖科技创新合作”(2130114)
广东省渔港建设和渔业产业发展专项“美丽硬仆骨舌鱼繁殖技术体系构建与示范”(A201701A07)
国家水产种质资源共享服务平台“珠江水系种质资源标准化整理、整合与共享”(2018DKA30470)
作者简介
第一作者简介:段永楠,男,1990年出生,研究生,研究方向:种质资源与城市渔业,通信地址:510380广州市荔湾区芳村西朗兴渔路1号珠江水产研究所,Tel:020-81517181,E-mail:1657133713@qq.com;通讯作者:牟希东,男,1981年出生,山东潍坊人,副研究员,研究生,硕士,主要从事种质资源与城市渔业,通信地址:510380广州市荔湾区芳村西朗兴渔路1号珠江水产研究所,Tel:020-81517181,E-mail:muxd1019@163.com。
