摘要
用8个微卫星标记组合建立了2个微卫星多重PCR体系,对大菱鲆7个人工选育家系进行了系谱认证、亲子鉴定和遗传多样性研究。2个多重PCR体系中8个微卫星位点共检测到等位基因49个,每个位点等位基因数为3~8个。根据已知亲本及子代基因型,成功推断出了3个缺失亲本的基因型。在双亲未知的情况下2个多重PCR的8个微卫星位点累积排除概率是96.58%,已知1个亲本时累积排除率为99.71%,亲子鉴定准确率为96.42%。采用70个个体进行双盲验证,利用UPGMA法对7个家系的70个体进行了聚类分析,同一家系95.71%的个体聚类分析结果与系谱来源一致。Cervus 2.0软件亲子鉴定结果表明亲子鉴定准确率为92.86%。2个多重PCR体系的建立为大菱鲆不同家系混养后的亲子鉴定、系谱分析和分子辅助家系管理提供了技术手段。
We developed two microsatellite multiplex PCR systems using 8 microsatellite loci for turbot（Scophthalmus maximus）,which is one important aquaculture species in China.The multiplex PCR microsatellite assay was used to estimate the kinship and the genetic diversity of seven turbot families.Based on the genotypes of either known parent or none of them and offspring,all the absent parental genotypes of the seven families were deduced.According to the unique alleles among the families,each family could be distinguished from the others.Cervus 2.0 analysis showed that the combined exclusion probability was 96.58% if parents were both unknown and the combined exclusion probability was 99.71% when only one of the parental genotype was known.The result 70 double-blind individuals showed that the combined exclusion probability and parentage analysis allocation rate was 95.72% and 92.86% by Cervus 2.0 analysis,respectively.The UPGAM dendrogram of the 70 individuals indicated that 95.71% of the individuals from the same family clusted together.The results showed that microsatellite multiplex PCR is an useful tool for parentage determination and genealogical identification of turbot.
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2011年第1期97-106,共10页
Periodical of Ocean University of China
基金
国家自然科学基金(30671623)
山东省泰山学者工程专项资助
关键词
大菱鲆
微卫星
多重PCR
亲子鉴定
系谱认证
turbot（Scophthalmus maximus）
microsatellite
multiplex PCR
parentage determination
genealogical identification
作者简介
苗贵东（1973-），男，博士生。Email：guidongmiao@126．com
通讯作者：Tel：0532-82031637；Email：tjfan@mail．ouc．edu．cn；
通讯作者：Tel：0532—85844606；Email：chensl@ysfri．ac．cn
