摘要
目的研究毛酸浆(Physalis pubescens L.)干燥茎叶的抗肿瘤化学成分。方法采用硅胶柱色谱、Sephadex LH-20柱色谱等多种色谱手段进行分离纯化,通过理化性质和多种波谱分析技术对分离得到的化合物进行结构鉴定。采用MTT法测定化合物对人肿瘤细胞的体外抑制增殖活性进行测试。结果从毛酸浆茎叶体积分数75%的乙醇溶液提取物中分离得到5个化合物,分别鉴定为(20S,22R,24S,25S,26Φ)-15α,16α-diacetoxy-5,6β:22,26-diepoxy-24-methoxy-4β,25,26(α/β)-trihydroxyergost-2-en-1-one(1)、physapubescin D(2)、(22E,24S)-5α,8α-epidioxy-24-methyl-cholesta-6,22-dien-3β-ol(3)、(22E,24S)-5α,8α-epidioxy-24-methyl-cholesta-6,9(11),22-trien-3β-ol(4)和alkesterol B(5)。化合物1对人前列腺癌细胞(C4-2B和22Rvl)、人肾癌细胞(786-O、Caki-2和ACHN)以及人黑色素瘤细胞(A375-S2)的IC50值分别为(5.10±1.36)、(1.0±0.38)、(0.48±0.14)、(0.67±0.39)、(9.20±2.77)和(2.83±0.52)μmol·L^(-1)。结论化合物1为新化合物,化合物3和4为首次从该属植物中分离得到。化合物1对选定的人肿瘤细胞具有较强的抑制增殖活性。
Objective To study the anti-tumor chemical constituents from Physalis pubescens L.Methods Compounds were isolated and purified by repeated column chromatography on silica gel column,Sephadex LH-20 column and preparative HPLC methods.Physicochemical properties characterization and spectroscopic methods were employed for the structure elucidation.Cytotoxic effects against six human cancer cell lines(C4-2B,22 Rvl,786-O,Caki-2,ACHN and A375-S2)were evaluated by MTT method.Results Five steroids were obtained and identified as (20 S,22R,24 S,25S,26Φ)-15α,16α-diacetoxy-5,6β: 22,26-diepoxy-24-methoxy-4β,25,26 (α/β)-trihydroxyergost-2-en-1-one (1),physapubescin D (2),(22 E,24S)-5α,8α-epidioxy-24-methyl-cholesta-6,22-dien-3β-ol (3),(22 E,24S)-5α,8α-epidioxy-24-methyl-cholesta-6,9(11),22-trien-3β-ol (4) and alkesterol B (5).Conclusions Compound 1 was a newwithanolide and compounds 3 and 4 were first isolated from the genus Physalis.Compound 1 exhibited moderate cytotoxicity against the tested cell lines with IC_(50) values in the range of 0.48-9.20 μmol·L^(-1).
出处
《沈阳药科大学学报》
CSCD
北大核心
2017年第9期757-762,共6页
Journal of Shenyang Pharmaceutical University
基金
国家自然科学基金资助项目(21472138
81430095
31270399)
关键词
毛酸浆
化学成分
结构鉴定
抗肿瘤
Physalis pubescens L.
chemical constituent
structure identification
anti-tumor
