摘要
为制备毛酸浆多糖泡腾颗粒剂并测定其抗氧化能力,本研究采取响应面法,筛选出泡腾颗粒剂的最佳处方,并对其进行质量评价。采用苯酚-浓硫酸检测法测定制剂中多糖的含量,并对其抗氧化能力进行测定。结果显示,最佳处方为15%的毛酸浆多糖粉末,碳酸氢钠与柠檬酸的质量比为1:1.1,聚乙二醇6000与碳酸氢钠的质量比为0.3:1,稀释剂为质量占比30%的甘露醇,黏合剂为6.4%的PVP-K30乙醇溶液。毛酸浆多糖泡腾颗粒有一定的还原力,其质量浓度与还原力呈正相关;在浓度为1.0mg/mL时,对·OH的清除率达到85.66%;在浓度为4.0 mg/mL时,对DPPH自由基的清除率达到83.45%;在浓度为4.0 mg/mL时,对超氧阴离子的清除率达到25.88%。毛酸浆多糖泡腾颗粒制剂服用方便、口味适宜,且具有良好的抗氧化活性。
The aim of this study is to prepare polysaccharides effervescent granules of Physalis pubescens and determine its antioxidant capacity.Response surface methodology was used to screen the best prescription of effervescent granules and evaluate its quality.The content of polysaccharide in the preparation was determined by phenol-concentrated sulfuric acid detection method.The antioxidant capacity was also measured.The results showed that the best prescription was 15% polysaccharide powder of pilose acid pulp,the mass ratio of sodium bicarbonate to citric acid was 1:1.1,the mass ratio of polyethylene glycol 6000 to sodium bicarbonate was 0.3:1,the diluent was mannitol with a mass ratio of 30%,the adhesive was PVP-K30 ethanol solution with a mass ratio of 6.4%.The polysaccharide effervescent granules of Physalis pubescens have certain reducing power,and its mass concentration is positively correlated with the reducing power;when the concentration is 1.0 mg/mL,the removal rate of ·OH reaches 85.66%;when the concentration is 4.0 mg/mL,the scavenging rate of DPPH free radical reaches 83.45%;when the concentration is 4.0 mg/mL,the removal rate of superoxide anion reaches 25.88%.The polysaccharide effervescent granules of Physalis pubescens is convenient to take,suitable in taste,and has good antioxidant activity.
作者
李春梅
张馨元
刘爽
史彤辉
周长胜
LI Chunmei;ZHANG Xinyuan;LIU Shuang;SHI Tonghui;ZHOU Changsheng(School of Pharmacy,Harbin University of Commerce,Harbin 150076,China;College of Marxism,Harbin University of Commerce,Harbin 150028,China)
出处
《特产研究》
2024年第1期37-44,49,共9页
Special Wild Economic Animal and Plant Research
基金
黑龙江省教育科学“十四五”规划省专项重点课题项目(GJE1422088)
2023年度黑龙江省中医药经典普及化专项课题(ZYW2023-077)
黑龙江省博士后科研启动金项目(BS0051)
哈尔滨商业大学博士科研启动项目(2019DS114)。
关键词
毛酸浆
多糖类
泡腾颗粒剂
抗氧化
Physalis pubescens
polysaccharides
effervescent granules
anti-oxidation
作者简介
李春梅(1981-),女,黑龙江克东人,博士,副教授,硕士生导师,主要从事天然产物研究开发与制剂研究;通讯作者:周长胜(1979-),男,吉林梨树人,博士,副教授,主要从事教育思政和中医药文化传承研究。
